Water Content in Nanoparticles Determined by Small-Angle Neutron Scattering and Light Scattering

Behrad Kangarlou; Donyeil Hoy; Haden L. Scott; Sai Venkatesh Pingali; Nora Khalil; Benjamin Chung; John Katsaras; Mu Ping Nieh

doi:10.1021/acs.langmuir.2c02420

Water Content in Nanoparticles Determined by Small-Angle Neutron Scattering and Light Scattering

Behrad Kangarlou, Donyeil Hoy, Haden L. Scott, Sai Venkatesh Pingali, Nora Khalil, Benjamin Chung, John Katsaras, Mu Ping Nieh

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The amount of water in therapeutic nanoparticles (NPs) is of great importance to the pharmaceutical industry, as water content reflects the volume occupied by the solid components. For example, certain biomolecules, such as mRNA, can undergo conformational change or degradation when exposed to water. Using static light scattering (SLS) and dynamic light scattering (DLS), we estimated the water content of NPs, including extruded liposomes of two different sizes and polystyrene (PS) Latex NPs. In addition, we used small-angle neutron scattering (SANS) to independently access the water content of the samples. The water content of NPs estimated by SLS/DLS was systematically higher than that from SANS. The discrepancy is most likely attributed to the larger radius determined by DLS, in contrast to the SANS-derived radius observed by SANS. However, because of low accessibility to the neutron facilities, we validate the combined SLS/DLS to be a reasonable alternative to SANS for determining the water (or solvent) content of NPs.

Original language	English
Pages (from-to)	227-235
Number of pages	9
Journal	Langmuir
Volume	39
Issue number	1
DOIs	https://doi.org/10.1021/acs.langmuir.2c02420
State	Published - Jan 10 2023

Funding

H.L.S. and J.K. were supported through the Scientific User Facilities Division of the DOE Office of Basic Energy Sciences under US DOE Contract No. DE-AC05-00OR22725. Neutron scattering studies performed on CG-3 Bio-SANS (IPTS-28955.1) and S.V.P are supported by the Office of Biological and Environmental Research funded Center for Structural Molecular Biology (CSMB) under Contract FWP ERKP291, using the High Flux Isotope Reactor supported by the Scientific User Facilities Division, Office of Basic Energy Sciences, U.S. Department of Energy. The SANS/SAXS data were fitted utilizing the SasView 4.2.2 application, originally developed under NSF award DMR-0520547. SasView contains code developed with funding from the European Union’s Horizon 2020 research and innovation programme under the SINE2020 project, grant agreement No. 654000. This manuscript has been coauthored by UT-Battelle, LLC, under Contract No. DE-AC05- 00OR22725 with the U.S. Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan ( http://energy.gov/downloads/doe-public-access-plan ). Acknowledgments

Funders	Funder number
Office of Biological and Environmental Research funded Center for Structural Molecular Biology
Scientific User Facilities Division
National Science Foundation	DMR-0520547
U.S. Department of Energy	DE-AC05-00OR22725, IPTS-28955.1
Basic Energy Sciences
Horizon 2020 Framework Programme	654000
Canadian Society for Molecular Biosciences	FWP ERKP291
UT-Battelle	DE-AC05- 00OR22725

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10.1021/acs.langmuir.2c02420

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abstract = "The amount of water in therapeutic nanoparticles (NPs) is of great importance to the pharmaceutical industry, as water content reflects the volume occupied by the solid components. For example, certain biomolecules, such as mRNA, can undergo conformational change or degradation when exposed to water. Using static light scattering (SLS) and dynamic light scattering (DLS), we estimated the water content of NPs, including extruded liposomes of two different sizes and polystyrene (PS) Latex NPs. In addition, we used small-angle neutron scattering (SANS) to independently access the water content of the samples. The water content of NPs estimated by SLS/DLS was systematically higher than that from SANS. The discrepancy is most likely attributed to the larger radius determined by DLS, in contrast to the SANS-derived radius observed by SANS. However, because of low accessibility to the neutron facilities, we validate the combined SLS/DLS to be a reasonable alternative to SANS for determining the water (or solvent) content of NPs.",

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AU - Katsaras, John

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Water Content in Nanoparticles Determined by Small-Angle Neutron Scattering and Light Scattering

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