Visualization of metabolic properties of bacterial cells using nanoscale secondary ion mass spectrometry (NanoSIMS)

Yi Vee Chew; Andrew J. Holmes; John B. Cliff

doi:10.1007/978-1-62703-712-9_11

Visualization of metabolic properties of bacterial cells using nanoscale secondary ion mass spectrometry (NanoSIMS)

Yi Vee Chew
, Andrew J. Holmes
, John B. Cliff

Applied Materials Analysis

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

5 Scopus citations

Abstract

NanoSIMS combines high-resolution imaging and mass spectrometry with simultaneous collection of up to seven different masses, providing an invaluable technique for determining the isotopic and elemental composition in microscopic target samples. It has been used in varying fields, from studying the elemental composition of mineral samples to tracking cell uptake of isotope-labelled substrates. In combination with in situ hybridization techniques, NanoSIMS offers a powerful method of linking metabolic capacity to phylogenetic identity in cell samples. Here, we describe methods and considerations for microbial sample preparation, visualization, and analysis using NanoSIMS.

Original language	English
Title of host publication	Environmental Microbiology
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press Inc.
Pages	133-146
Number of pages	14
ISBN (Print)	9781627037112
DOIs	https://doi.org/10.1007/978-1-62703-712-9_11
State	Published - 2014

Publication series

Name	Methods in Molecular Biology
Volume	1096
ISSN (Print)	1064-3745

Keywords

Cell imaging
Elemental tracking
Fluorescence in situ hybridization (FISH)
Halogenous molecules
Isotope tracking
NanoSIMS
Stable isotope enrichment
Subcellular localization

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10.1007/978-1-62703-712-9_11

Cite this

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abstract = "NanoSIMS combines high-resolution imaging and mass spectrometry with simultaneous collection of up to seven different masses, providing an invaluable technique for determining the isotopic and elemental composition in microscopic target samples. It has been used in varying fields, from studying the elemental composition of mineral samples to tracking cell uptake of isotope-labelled substrates. In combination with in situ hybridization techniques, NanoSIMS offers a powerful method of linking metabolic capacity to phylogenetic identity in cell samples. Here, we describe methods and considerations for microbial sample preparation, visualization, and analysis using NanoSIMS.",

keywords = "Cell imaging, Elemental tracking, Fluorescence in situ hybridization (FISH), Halogenous molecules, Isotope tracking, NanoSIMS, Stable isotope enrichment, Subcellular localization",

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language = "English",

isbn = "9781627037112",

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Visualization of metabolic properties of bacterial cells using nanoscale secondary ion mass spectrometry (NanoSIMS). / Chew, Yi Vee; Holmes, Andrew J.; Cliff, John B.
Environmental Microbiology: Methods and Protocols. Humana Press Inc., 2014. p. 133-146 (Methods in Molecular Biology; Vol. 1096).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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T1 - Visualization of metabolic properties of bacterial cells using nanoscale secondary ion mass spectrometry (NanoSIMS)

AU - Chew, Yi Vee

AU - Holmes, Andrew J.

AU - Cliff, John B.

PY - 2014

Y1 - 2014

N2 - NanoSIMS combines high-resolution imaging and mass spectrometry with simultaneous collection of up to seven different masses, providing an invaluable technique for determining the isotopic and elemental composition in microscopic target samples. It has been used in varying fields, from studying the elemental composition of mineral samples to tracking cell uptake of isotope-labelled substrates. In combination with in situ hybridization techniques, NanoSIMS offers a powerful method of linking metabolic capacity to phylogenetic identity in cell samples. Here, we describe methods and considerations for microbial sample preparation, visualization, and analysis using NanoSIMS.

AB - NanoSIMS combines high-resolution imaging and mass spectrometry with simultaneous collection of up to seven different masses, providing an invaluable technique for determining the isotopic and elemental composition in microscopic target samples. It has been used in varying fields, from studying the elemental composition of mineral samples to tracking cell uptake of isotope-labelled substrates. In combination with in situ hybridization techniques, NanoSIMS offers a powerful method of linking metabolic capacity to phylogenetic identity in cell samples. Here, we describe methods and considerations for microbial sample preparation, visualization, and analysis using NanoSIMS.

KW - Cell imaging

KW - Elemental tracking

KW - Fluorescence in situ hybridization (FISH)

KW - Halogenous molecules

KW - Isotope tracking

KW - NanoSIMS

KW - Stable isotope enrichment

KW - Subcellular localization

UR - https://www.scopus.com/pages/publications/84907601706

U2 - 10.1007/978-1-62703-712-9_11

DO - 10.1007/978-1-62703-712-9_11

M3 - Chapter

C2 - 24515366

AN - SCOPUS:84907601706

SN - 9781627037112

T3 - Methods in Molecular Biology

SP - 133

EP - 146

BT - Environmental Microbiology

PB - Humana Press Inc.

ER -

Visualization of metabolic properties of bacterial cells using nanoscale secondary ion mass spectrometry (NanoSIMS)

Abstract

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Keywords

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