TY - JOUR
T1 - UV-Vis Spectrophotometric Analysis and Quantification of Glyphosate for an Interdisciplinary Undergraduate Laboratory
AU - Felton, Daniel E.
AU - Ederer, Martina
AU - Steffens, Timothy
AU - Hartzell, Patricia L.
AU - Waynant, Kristopher V.
N1 - Publisher Copyright:
© 2017 The American Chemical Society and Division of Chemical Education, Inc.
PY - 2018/1/9
Y1 - 2018/1/9
N2 - Glyphosate (N-(phosphonomethyl)glycine) is the most widely used herbicide on earth. A simple assay to quantify glyphosate concentrations in environmental samples was developed as part of an interdisciplinary effort linking introductory laboratory courses in chemistry, biology, and microbiology. In this 3 h laboratory experiment, students used UV-vis spectroscopy to quantify glyphosate in prepared unknowns and supernatants from glyphosate-treated soil samples. Regression analysis indicated that the assay is linear up to 20.0 ppm, making it particularly useful for detection of low levels of glyphosate in environmental samples. The assay can be used to quantify the activity of glyphosate-degrading soil microorganisms by comparing glyphosate levels between autoclaved and nonautoclaved soil slurries.
AB - Glyphosate (N-(phosphonomethyl)glycine) is the most widely used herbicide on earth. A simple assay to quantify glyphosate concentrations in environmental samples was developed as part of an interdisciplinary effort linking introductory laboratory courses in chemistry, biology, and microbiology. In this 3 h laboratory experiment, students used UV-vis spectroscopy to quantify glyphosate in prepared unknowns and supernatants from glyphosate-treated soil samples. Regression analysis indicated that the assay is linear up to 20.0 ppm, making it particularly useful for detection of low levels of glyphosate in environmental samples. The assay can be used to quantify the activity of glyphosate-degrading soil microorganisms by comparing glyphosate levels between autoclaved and nonautoclaved soil slurries.
KW - First-Year Undergraduate/General
KW - Inquiry-Based/Discovery Learning
KW - Interdisciplinary/Multidisciplinary
KW - Laboratory Instruction
KW - UV-Vis Spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85040354834&partnerID=8YFLogxK
U2 - 10.1021/acs.jchemed.7b00440
DO - 10.1021/acs.jchemed.7b00440
M3 - Article
AN - SCOPUS:85040354834
SN - 0021-9584
VL - 95
SP - 136
EP - 140
JO - Journal of Chemical Education
JF - Journal of Chemical Education
IS - 1
ER -