Ultra-thin layer chromatography with integrated silver colloid-based SERS detection

Ryan A. Wallace, Nickolay V. Lavrik, Michael J. Sepaniak

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Simplified lab-on-a-chip techniques are desirable for quick and efficient detection of analytes of interest in the field. The following work involves the use of deterministic pillar arrays on the micro-scale as a platform to separate compounds, and the use of Ag colloid within the arrays as a source of increased signal via surface enhanced Raman spectroscopy (SERS). One problem traditionally seen with SERS surfaces containing Ag colloid is oxidation; however, our platforms are superhydrophobic, reducing the amount of oxidation taking place on the surface of the Ag colloid. This work includes the successful separation and SERS detection of a fluorescent dye compounds (resorufin and sulforhodamine 640), fluorescent anti-tumor drugs (Adriamycin and Daunomycin), and purine and pyrimidine bases (adenine, cytosine, guanine, hypoxanthine, and thymine).

Original languageEnglish
Pages (from-to)361-367
Number of pages7
JournalElectrophoresis
Volume38
Issue number2
DOIs
StatePublished - Jan 1 2017

Funding

All authors have given approval to the final version of the manuscript. This material is based upon work supported by the National Science Foundation under Grant CHE-1144947 with the University of Tennessee. A portion of this research was conducted at the Center for Nanophase Materials Sciences, which is sponsored at Oak Ridge National Laboratory by the Scientific User Facilities Division, Office of Basic Energy Sciences, U.S. Department of Energy.

Keywords

  • Photolithography
  • SERS
  • Ultra-thin layer chromatograpy

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