Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry

Xin Hua; Matthew J. Marshall; Yijia Xiong; Xiang Ma; Yufan Zhou; Abigail E. Tucker; Zihua Zhu; Songqin Liu; Xiao Ying Yu

doi:10.1063/1.4919807

Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry

Xin Hua, Matthew J. Marshall, Yijia Xiong, Xiang Ma, Yufan Zhou, Abigail E. Tucker, Zihua Zhu, Songqin Liu, Xiao Ying Yu

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

A vacuum compatible microfluidic reactor, SALVI (System for Analysis at the Liquid Vacuum Interface), was employed for in situ chemical imaging of live biofilms using time-of-flight secondary ion mass spectrometry (ToF-SIMS). Depth profiling by sputtering materials in sequential layers resulted in live biofilm spatial chemical mapping. Two-dimensional (2D) images were reconstructed to report the first three-dimensional images of hydrated biofilm elucidating spatial and chemical heterogeneity. 2D image principal component analysis was conducted among biofilms at different locations in the microchannel. Our approach directly visualized spatial and chemical heterogeneity within the living biofilm by dynamic liquid ToF-SIMS.

Original language	English
Article number	031101
Journal	Biomicrofluidics
Volume	9
Issue number	3
DOIs	https://doi.org/10.1063/1.4919807
State	Published - May 5 2015
Externally published	Yes

Access to Document

10.1063/1.4919807

Cite this

@article{82c697f2ea7b4d6d99dcfc7cd5f95d9a,

title = "Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry",

abstract = "A vacuum compatible microfluidic reactor, SALVI (System for Analysis at the Liquid Vacuum Interface), was employed for in situ chemical imaging of live biofilms using time-of-flight secondary ion mass spectrometry (ToF-SIMS). Depth profiling by sputtering materials in sequential layers resulted in live biofilm spatial chemical mapping. Two-dimensional (2D) images were reconstructed to report the first three-dimensional images of hydrated biofilm elucidating spatial and chemical heterogeneity. 2D image principal component analysis was conducted among biofilms at different locations in the microchannel. Our approach directly visualized spatial and chemical heterogeneity within the living biofilm by dynamic liquid ToF-SIMS.",

author = "Xin Hua and Marshall, {Matthew J.} and Yijia Xiong and Xiang Ma and Yufan Zhou and Tucker, {Abigail E.} and Zihua Zhu and Songqin Liu and Yu, {Xiao Ying}",

note = "Publisher Copyright: {\textcopyright} 2015 AIP Publishing LLC.",

year = "2015",

month = may,

day = "5",

doi = "10.1063/1.4919807",

language = "English",

volume = "9",

journal = "Biomicrofluidics",

issn = "1932-1058",

publisher = "American Institute of Physics",

number = "3",

}

TY - JOUR

T1 - Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry

AU - Hua, Xin

AU - Marshall, Matthew J.

AU - Xiong, Yijia

AU - Ma, Xiang

AU - Zhou, Yufan

AU - Tucker, Abigail E.

AU - Zhu, Zihua

AU - Liu, Songqin

AU - Yu, Xiao Ying

PY - 2015/5/5

Y1 - 2015/5/5

N2 - A vacuum compatible microfluidic reactor, SALVI (System for Analysis at the Liquid Vacuum Interface), was employed for in situ chemical imaging of live biofilms using time-of-flight secondary ion mass spectrometry (ToF-SIMS). Depth profiling by sputtering materials in sequential layers resulted in live biofilm spatial chemical mapping. Two-dimensional (2D) images were reconstructed to report the first three-dimensional images of hydrated biofilm elucidating spatial and chemical heterogeneity. 2D image principal component analysis was conducted among biofilms at different locations in the microchannel. Our approach directly visualized spatial and chemical heterogeneity within the living biofilm by dynamic liquid ToF-SIMS.

AB - A vacuum compatible microfluidic reactor, SALVI (System for Analysis at the Liquid Vacuum Interface), was employed for in situ chemical imaging of live biofilms using time-of-flight secondary ion mass spectrometry (ToF-SIMS). Depth profiling by sputtering materials in sequential layers resulted in live biofilm spatial chemical mapping. Two-dimensional (2D) images were reconstructed to report the first three-dimensional images of hydrated biofilm elucidating spatial and chemical heterogeneity. 2D image principal component analysis was conducted among biofilms at different locations in the microchannel. Our approach directly visualized spatial and chemical heterogeneity within the living biofilm by dynamic liquid ToF-SIMS.

UR - http://www.scopus.com/inward/record.url?scp=84928914669&partnerID=8YFLogxK

U2 - 10.1063/1.4919807

DO - 10.1063/1.4919807

M3 - Article

AN - SCOPUS:84928914669

SN - 1932-1058

VL - 9

JO - Biomicrofluidics

JF - Biomicrofluidics

IS - 3

M1 - 031101

ER -

Two-dimensional and three-dimensional dynamic imaging of live biofilms in a microchannel by time-of-flight secondary ion mass spectrometry

Abstract

Access to Document

Other files and links

Fingerprint

Cite this