Transfer of sulfur to biotin from biotin synthase (BioB protein)

Katharine J. Gibson, Dale A. Pelletier, Ivan M. Turner

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

In biotin synthase reactions carried out in vitro, we observed efficient transfer of 35S to biotin from partially purified E. coli biotin synthase (product of the bioB gene) labelled by biosynthetic incorporation of [35S]-cysteine. Mass spectrometry was consistent with covalent alteration of the protein in the assay. These results suggest that BioB protein is a reagent, not a catalyst.

Original languageEnglish
Pages (from-to)632-635
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume254
Issue number3
DOIs
StatePublished - Jan 27 1999
Externally publishedYes

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