TY - JOUR
T1 - Transfer of sulfur to biotin from biotin synthase (BioB protein)
AU - Gibson, Katharine J.
AU - Pelletier, Dale A.
AU - Turner, Ivan M.
PY - 1999/1/27
Y1 - 1999/1/27
N2 - In biotin synthase reactions carried out in vitro, we observed efficient transfer of 35S to biotin from partially purified E. coli biotin synthase (product of the bioB gene) labelled by biosynthetic incorporation of [35S]-cysteine. Mass spectrometry was consistent with covalent alteration of the protein in the assay. These results suggest that BioB protein is a reagent, not a catalyst.
AB - In biotin synthase reactions carried out in vitro, we observed efficient transfer of 35S to biotin from partially purified E. coli biotin synthase (product of the bioB gene) labelled by biosynthetic incorporation of [35S]-cysteine. Mass spectrometry was consistent with covalent alteration of the protein in the assay. These results suggest that BioB protein is a reagent, not a catalyst.
UR - http://www.scopus.com/inward/record.url?scp=0033608163&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1998.9991
DO - 10.1006/bbrc.1998.9991
M3 - Article
C2 - 9920791
AN - SCOPUS:0033608163
SN - 0006-291X
VL - 254
SP - 632
EP - 635
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -