Towards biochemical filters with a sigmoidal response to pH changes: Buffered biocatalytic signal transduction

Marcos Pita, Vladimir Privman, Mary A. Arugula, Dmitriy Melnikov, Vera Bocharova, Evgeny Katz

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

We realize a biochemical filtering process by introducing a buffer in a biocatalytic signal-transduction logic system based on the function of an enzyme, esterase. The input, ethyl butyrate, is converted into butyric acid - the output signal, which in turn is measured by the drop in the pH value. The developed approach offers a versatile "network element" for increasing the complexity of biochemical information processing systems. Evaluation of an optimal regime for quality filtering is accomplished in the framework of a kinetic rate-equation model.

Original languageEnglish
Pages (from-to)4507-4513
Number of pages7
JournalPhysical Chemistry Chemical Physics
Volume13
Issue number10
DOIs
StatePublished - Mar 14 2011
Externally publishedYes

Funding

FundersFunder number
National Science Foundation1015983

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