Total internal reflection enabled wide-field coherent anti-Stokes Raman scattering microscopy

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6 Scopus citations

Abstract

Wide-field coherent anti-Stokes Raman scattering (CARS) microscopy offers an attractive means for the rapid and simultaneous acquisition of vibrationally resolved images across a large field of view. A major challenge in the implementation lies in how to achieve sufficiently strong excitation fields necessary to drive the third-order optical responses over the large focal region. Here, we report a new wide-field CARS microscope enabled by a total internal reflection excitation scheme using a femtosecond Ti:Sapphire oscillator to generate pump and broadband near-infrared Stokes pulses. The spectrally broad Stokes pulse, in combination with its inherent chirp, offers not only access to a wide range of Raman modes spanning ∼1000 to ∼3500 cm−1 but also a straightforward means to select vibrational transitions within this range by simply varying the time delay between the pulses. The unique capabilities of this wide-field CARS microscope were validated by acquiring high-quality CARS images from the model and complex biological samples on conventional microscope coverslips.

Original languageEnglish
Pages (from-to)3087-3090
Number of pages4
JournalOptics Letters
Volume45
Issue number11
DOIs
StatePublished - Jun 1 2020

Funding

Acknowledgment. Research was supported by U.S. Department of Energy, Office of Science, Biological and Environmental Research, Bioimaging Science Program. The microfluidic cells used in this experiment were fabricated in the Nanofabrication Research Lab at the Center for Nanophase Materials Sciences, which is a DOE Office of Science User Facility. B.D. would like to acknowledge useful conversations with Prof. Tessa R. Calhoun and Dr. Brianna R. Watson regarding the design of this microscope. Biological and Environmental Research; Office of Science; U.S. Department of Energy. Research was supported by U.S. Department of Energy, Office of Science, Biological and Environmental Research, Bioimaging Science Program. The microfluidic cells used in this experiment were fabricated in the Nanofabrication Research Lab at the Center for Nanophase Materials Sciences, which is a DOE Office of Science User Facility. B.D. would like to acknowledge useful conversations with Prof. Tessa R. Calhoun and Dr. Brianna R. Watson regarding the design of this microscope.

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