Abstract
Analysis of bacterial biofilms is particularly challenging and important with diverse applications from systems biology to biotechnology. Among the variety of techniques that have been applied, time-of-flight secondary ion mass spectrometry (ToF-SIMS) has many powerful features in studying the surface characteristics of biofilms. ToF-SIMS offers high spatial resolution, mass resolution, and mass accuracy, which permit surface sensitive analysis of biofilm components. Thus, ToF-SIMS provides a powerful solution to addressing the challenge of bacterial biofilm analysis. This dataset covers ToF-SIMS analysis of Shewanella oneidensis MR-1 isolated from freshwater lake sediment in New York state. The MR-1 strain is known to have metal and sulfur reducing properties and it can be used for bioremediation and wastewater treatment. There is a current need to identify small molecules and fragments produced from bacterial biofilms, especially those from extracellular polymeric substance (EPS). Static ToF-SIMS spectra of MR-1 were obtained using an IONTOF TOF.SIMS V instrument equipped with a 25 keV Bi3+ metal ion gun. Identified molecules and molecular fragments are compared against known biological databases and the reported peaks have at least 65 ppm mass accuracy. These molecules range from lipids, fatty acids, flavonoids, and quinolones to other naturally occurring organic compounds. It is anticipated that the mass spectral identification of key peaks will assist detection of metabolites, EPS molecules like polysaccharides, and biologically relevant small organic molecules using ToF-SIMS in future surface and interface research.
| Original language | English |
|---|---|
| Article number | 100396 |
| Journal | Results in Surfaces and Interfaces |
| Volume | 18 |
| DOIs | |
| State | Published - Jan 2025 |
Funding
Gabriel Parker was funded by the Department of Energy SCGSR fellowship program and the GRO internship program at Oak Ridge National Laboratory. The authors would like to thank Dr. Yuchen Zhang, Dr. Jiyoung Son, and Rachel Komorek for their contribution for the sample analysis and data collection. Dr. Xiao-Ying Yu is indebted to the support of the strategic Laboratory Directed Research and Development of the Physical Sciences Directorate of the Oak Ridge National Laboratory (ORNL). This manuscript has been authored by UT-Battelle, LLC under Contract No. DE-AC05-00OR22725 with the U.S. DOE. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, worldwide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The DOE will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan).
Keywords
- Biofilms
- extracellular polymeric substance
- Fatty acids
- Metabolites
- Shewanella oneidensis MR-1
- Time-of-flight secondary ion mass spectrometry
