Time-of-flight Bragg scattering from aligned stacks of lipid bilayers using the Liquids Reflectometer at the Spallation Neutron Source

Jianjun Pan, Frederick A. Heberle, Justin R. Carmichael, John F. Ankner, John Katsaras

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Time-of-flight (TOF) neutron diffraction experiments on aligned stacks of lipid bilayers using the horizontal Liquids Reflectometer at the Spallation Neutron Source are reported. Specific details are given regarding the instrumental setup, data collection and reduction, phase determination of the structure factors, and reconstruction of the one-dimensional neutron scattering length density (NSLD) profile. The validity of using TOF measurements to determine the one-dimensional NSLD profile is demonstrated by reproducing the results of two well known lipid bilayer structures. The method is then applied to show how an antimicrobial peptide affects membranes with and without cholesterol.

Original languageEnglish
Pages (from-to)1219-1227
Number of pages9
JournalJournal of Applied Crystallography
Volume45
Issue number6
DOIs
StatePublished - Dec 2012

Keywords

  • Liquids Reflectometer
  • Spallation Neutron Source
  • lipid bilayers
  • time-of-flight neutron diffraction

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