TY - JOUR
T1 - Time-of-flight Bragg scattering from aligned stacks of lipid bilayers using the Liquids Reflectometer at the Spallation Neutron Source
AU - Pan, Jianjun
AU - Heberle, Frederick A.
AU - Carmichael, Justin R.
AU - Ankner, John F.
AU - Katsaras, John
PY - 2012/12
Y1 - 2012/12
N2 - Time-of-flight (TOF) neutron diffraction experiments on aligned stacks of lipid bilayers using the horizontal Liquids Reflectometer at the Spallation Neutron Source are reported. Specific details are given regarding the instrumental setup, data collection and reduction, phase determination of the structure factors, and reconstruction of the one-dimensional neutron scattering length density (NSLD) profile. The validity of using TOF measurements to determine the one-dimensional NSLD profile is demonstrated by reproducing the results of two well known lipid bilayer structures. The method is then applied to show how an antimicrobial peptide affects membranes with and without cholesterol.
AB - Time-of-flight (TOF) neutron diffraction experiments on aligned stacks of lipid bilayers using the horizontal Liquids Reflectometer at the Spallation Neutron Source are reported. Specific details are given regarding the instrumental setup, data collection and reduction, phase determination of the structure factors, and reconstruction of the one-dimensional neutron scattering length density (NSLD) profile. The validity of using TOF measurements to determine the one-dimensional NSLD profile is demonstrated by reproducing the results of two well known lipid bilayer structures. The method is then applied to show how an antimicrobial peptide affects membranes with and without cholesterol.
KW - Liquids Reflectometer
KW - Spallation Neutron Source
KW - lipid bilayers
KW - time-of-flight neutron diffraction
UR - http://www.scopus.com/inward/record.url?scp=84869194234&partnerID=8YFLogxK
U2 - 10.1107/S0021889812039945
DO - 10.1107/S0021889812039945
M3 - Article
AN - SCOPUS:84869194234
SN - 0021-8898
VL - 45
SP - 1219
EP - 1227
JO - Journal of Applied Crystallography
JF - Journal of Applied Crystallography
IS - 6
ER -