Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking

Seth T. Langford; Cody S. Wiggins; Roque Santos; Melinda Hauser; Jeffrey M. Becker; Arthur E. Ruggles

doi:10.1371/journal.pone.0180503

Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking

Seth T. Langford, Cody S. Wiggins, Roque Santos, Melinda Hauser, Jeffrey M. Becker, Arthur E. Ruggles

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. A population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by ¹⁸F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. This work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.

Original language	English
Article number	e0180503
Journal	PLoS ONE
Volume	12
Issue number	7
DOIs	https://doi.org/10.1371/journal.pone.0180503
State	Published - Jul 2017
Externally published	Yes

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title = "Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking",

abstract = "A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. A population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by 18F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. This work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.",

author = "Langford, {Seth T.} and Wiggins, {Cody S.} and Roque Santos and Melinda Hauser and Becker, {Jeffrey M.} and Ruggles, {Arthur E.}",

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AU - Wiggins, Cody S.

AU - Santos, Roque

AU - Hauser, Melinda

AU - Becker, Jeffrey M.

AU - Ruggles, Arthur E.

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Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking

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