Thermodesulfobacterium geofontis sp. nov., a hyperthermophilic, sulfate-reducing bacterium isolated from Obsidian Pool, Yellowstone National Park

Scott D. Hamilton-Brehm; Robert A. Gibson; Stefan J. Green; Ellen C. Hopmans; Stefan Schouten; Marcel T.J. van der Meer; John P. Shields; Jaap S.S. Damsté; James G. Elkins

doi:10.1007/s00792-013-0512-1

Thermodesulfobacterium geofontis sp. nov., a hyperthermophilic, sulfate-reducing bacterium isolated from Obsidian Pool, Yellowstone National Park

Scott D. Hamilton-Brehm, Robert A. Gibson, Stefan J. Green, Ellen C. Hopmans, Stefan Schouten, Marcel T.J. van der Meer, John P. Shields, Jaap S.S. Damsté, James G. Elkins

Synthetic Biology

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

A novel sulfate-reducing bacterium designated OPF15^T was isolated from Obsidian Pool, Yellowstone National Park, Wyoming. The phylogeny of 16S rRNA and functional genes (dsrAB) placed the organism within the family Thermodesulfobacteriaceae. The organism displayed hyperthermophilic temperature requirements for growth with a range of 70-90 °C and an optimum of 83 °C. Optimal pH was around 6. 5-7. 0 and the organism required the presence of H₂ or formate as an electron donor and CO₂ as a carbon source. Electron acceptors supporting growth included sulfate, thiosulfate, and elemental sulfur. Lactate, acetate, pyruvate, benzoate, oleic acid, and ethanol did not serve as electron donors. Membrane lipid analysis revealed diacyl glycerols and acyl/ether glycerols which ranged from C_14:0 to C_20:0. Alkyl chains present in acyl/ether and diether glycerol lipids ranged from C_16:0 to C_18:0. Straight, iso- and anteiso-configurations were found for all lipid types. The presence of OPF15^T was also shown to increase cellulose consumption during co-cultivation with Caldicellulosiruptor obsidiansis, a fermentative, cellulolytic extreme thermophile isolated from the same environment. On the basis of phylogenetic, phenotypic, and structural analyses, Thermodesulfobacterium geofontis sp. nov. is proposed as a new species with OPF15^T representing the type strain.

Original language	English
Pages (from-to)	251-263
Number of pages	13
Journal	Extremophiles
Volume	17
Issue number	2
DOIs	https://doi.org/10.1007/s00792-013-0512-1
State	Published - Mar 2013

Funding

Acknowledgments We thank Sarah J. Kauffman for her invaluable technical assistance. We also thank Brian P. Hedlund, Christie Hendrix, and the National Park Service for sample collection under permit #YELL-SCI-0115 (PI: Karl O. Stetter). Christopher W. Schadt and Dwayne A. Elias provided helpful comments during preparation of the manuscript. Support for S. D. H.-B. and J. G. E. was provided by the BioEnergy Science Center (BESC), which is a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science, Oak Ridge National Laboratory. Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the U.S. Department of Energy under contract DE-AC05-00OR22725. The research was partially funded by the Netherlands Darwin Centre for Biogeosciences by a grant for a post-doctoral fellowship (R.A.G.) to J.S.S.D.

Funders	Funder number
BioEnergy Science Center
Netherlands Darwin Centre for Biogeosciences
U.S. Department of Energy Bioenergy Research Center
U.S. Department of Energy	DE-AC05-00OR22725
Biological and Environmental Research
Oak Ridge National Laboratory

Keywords

Dissimilatory sulfate reduction
Hyperthermophile
Membrane lipids
Thermal environments
Thermodesulfobacterium

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10.1007/s00792-013-0512-1

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Hamilton-Brehm, S. D., Gibson, R. A., Green, S. J., Hopmans, E. C., Schouten, S., van der Meer, M. T. J., Shields, J. P., Damsté, J. S. S., & Elkins, J. G. (2013). Thermodesulfobacterium geofontis sp. nov., a hyperthermophilic, sulfate-reducing bacterium isolated from Obsidian Pool, Yellowstone National Park. Extremophiles, 17(2), 251-263. https://doi.org/10.1007/s00792-013-0512-1

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abstract = "A novel sulfate-reducing bacterium designated OPF15T was isolated from Obsidian Pool, Yellowstone National Park, Wyoming. The phylogeny of 16S rRNA and functional genes (dsrAB) placed the organism within the family Thermodesulfobacteriaceae. The organism displayed hyperthermophilic temperature requirements for growth with a range of 70-90 °C and an optimum of 83 °C. Optimal pH was around 6. 5-7. 0 and the organism required the presence of H2 or formate as an electron donor and CO2 as a carbon source. Electron acceptors supporting growth included sulfate, thiosulfate, and elemental sulfur. Lactate, acetate, pyruvate, benzoate, oleic acid, and ethanol did not serve as electron donors. Membrane lipid analysis revealed diacyl glycerols and acyl/ether glycerols which ranged from C14:0 to C20:0. Alkyl chains present in acyl/ether and diether glycerol lipids ranged from C16:0 to C18:0. Straight, iso- and anteiso-configurations were found for all lipid types. The presence of OPF15T was also shown to increase cellulose consumption during co-cultivation with Caldicellulosiruptor obsidiansis, a fermentative, cellulolytic extreme thermophile isolated from the same environment. On the basis of phylogenetic, phenotypic, and structural analyses, Thermodesulfobacterium geofontis sp. nov. is proposed as a new species with OPF15T representing the type strain.",

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AU - Hamilton-Brehm, Scott D.

AU - Gibson, Robert A.

AU - Green, Stefan J.

AU - Hopmans, Ellen C.

AU - Schouten, Stefan

AU - van der Meer, Marcel T.J.

AU - Shields, John P.

AU - Damsté, Jaap S.S.

AU - Elkins, James G.

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N2 - A novel sulfate-reducing bacterium designated OPF15T was isolated from Obsidian Pool, Yellowstone National Park, Wyoming. The phylogeny of 16S rRNA and functional genes (dsrAB) placed the organism within the family Thermodesulfobacteriaceae. The organism displayed hyperthermophilic temperature requirements for growth with a range of 70-90 °C and an optimum of 83 °C. Optimal pH was around 6. 5-7. 0 and the organism required the presence of H2 or formate as an electron donor and CO2 as a carbon source. Electron acceptors supporting growth included sulfate, thiosulfate, and elemental sulfur. Lactate, acetate, pyruvate, benzoate, oleic acid, and ethanol did not serve as electron donors. Membrane lipid analysis revealed diacyl glycerols and acyl/ether glycerols which ranged from C14:0 to C20:0. Alkyl chains present in acyl/ether and diether glycerol lipids ranged from C16:0 to C18:0. Straight, iso- and anteiso-configurations were found for all lipid types. The presence of OPF15T was also shown to increase cellulose consumption during co-cultivation with Caldicellulosiruptor obsidiansis, a fermentative, cellulolytic extreme thermophile isolated from the same environment. On the basis of phylogenetic, phenotypic, and structural analyses, Thermodesulfobacterium geofontis sp. nov. is proposed as a new species with OPF15T representing the type strain.

AB - A novel sulfate-reducing bacterium designated OPF15T was isolated from Obsidian Pool, Yellowstone National Park, Wyoming. The phylogeny of 16S rRNA and functional genes (dsrAB) placed the organism within the family Thermodesulfobacteriaceae. The organism displayed hyperthermophilic temperature requirements for growth with a range of 70-90 °C and an optimum of 83 °C. Optimal pH was around 6. 5-7. 0 and the organism required the presence of H2 or formate as an electron donor and CO2 as a carbon source. Electron acceptors supporting growth included sulfate, thiosulfate, and elemental sulfur. Lactate, acetate, pyruvate, benzoate, oleic acid, and ethanol did not serve as electron donors. Membrane lipid analysis revealed diacyl glycerols and acyl/ether glycerols which ranged from C14:0 to C20:0. Alkyl chains present in acyl/ether and diether glycerol lipids ranged from C16:0 to C18:0. Straight, iso- and anteiso-configurations were found for all lipid types. The presence of OPF15T was also shown to increase cellulose consumption during co-cultivation with Caldicellulosiruptor obsidiansis, a fermentative, cellulolytic extreme thermophile isolated from the same environment. On the basis of phylogenetic, phenotypic, and structural analyses, Thermodesulfobacterium geofontis sp. nov. is proposed as a new species with OPF15T representing the type strain.

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KW - Membrane lipids

KW - Thermal environments

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Thermodesulfobacterium geofontis sp. nov., a hyperthermophilic, sulfate-reducing bacterium isolated from Obsidian Pool, Yellowstone National Park

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