The X-ray crystal structure of Shewanella oneidensis OmcA reveals new insight at the microbe-mineral interface

Marcus J. Edwards; Nanakow A. Baiden; Alexander Johs; Stephen J. Tomanicek; Liyuan Liang; Liang Shi; Jim K. Fredrickson; John M. Zachara; Andrew J. Gates; Julea N. Butt; David J. Richardson; Thomas A. Clarke

doi:10.1016/j.febslet.2014.04.013

The X-ray crystal structure of Shewanella oneidensis OmcA reveals new insight at the microbe-mineral interface

Marcus J. Edwards, Nanakow A. Baiden, Alexander Johs, Stephen J. Tomanicek, Liyuan Liang, Liang Shi, Jim K. Fredrickson, John M. Zachara, Andrew J. Gates, Julea N. Butt, David J. Richardson, Thomas A. Clarke

Biogeochemical Dynamics

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71 Scopus citations

Abstract

The X-ray crystal structure of Shewanella oneidensis OmcA, an extracellular decaheme cytochrome involved in mineral reduction, was solved to a resolution of 2.7 Å. The four OmcA molecules in the asymmetric unit are arranged so the minimum distance between heme 5 on adjacent OmcA monomers is 9 Å, indicative of a transient OmcA dimer capable of intermolecular electron transfer. A previously identified hematite binding motif was identified near heme 10, forming a hydroxylated surface that would bring a heme 10 electron egress site to ∼10 Å of a mineral surface.

Original language	English
Pages (from-to)	1886-1890
Number of pages	5
Journal	FEBS Letters
Volume	588
Issue number	10
DOIs	https://doi.org/10.1016/j.febslet.2014.04.013
State	Published - May 21 2014

Funding

DJR is a Royal Society Wolfson Foundation Merit Award holder. This research was supported by the Biotechnology and Biological Sciences Research Council ( BB/K00929X/1 and BB/H007288/1 ) and sponsored by the Subsurface Biogeochemical Research program (SBR)/Office of Biological and Environmental Research (BER) , U.S. Department of Energy (DOE) , and is a contribution of the Pacific Northwest National Laboratory (PNNL) Scientific Focus Area and the Mercury Scientific Focus Area at Oak Ridge National Laboratory (ORNL). The PNNL and ORNL are operated for the DOE by Battelle under contracts DE-AC05-76RLO1830 and DE-AC05-00OR22725

Funders	Funder number
U.S. Department of Energy
Biological and Environmental Research
Oak Ridge National Laboratory
Pacific Northwest National Laboratory
Biotechnology and Biological Sciences Research Council	BB/H007288/1, BB/K00929X/1

Keywords

Electron transfer
Metalloprotein
Mineral respiration
Multiheme cytochrome
Outer membrane
Shewanella
c-Type heme

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10.1016/j.febslet.2014.04.013

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Edwards, M. J., Baiden, N. A., Johs, A., Tomanicek, S. J., Liang, L., Shi, L., Fredrickson, J. K., Zachara, J. M., Gates, A. J., Butt, J. N., Richardson, D. J., & Clarke, T. A. (2014). The X-ray crystal structure of Shewanella oneidensis OmcA reveals new insight at the microbe-mineral interface. FEBS Letters, 588(10), 1886-1890. https://doi.org/10.1016/j.febslet.2014.04.013

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abstract = "The X-ray crystal structure of Shewanella oneidensis OmcA, an extracellular decaheme cytochrome involved in mineral reduction, was solved to a resolution of 2.7 {\AA}. The four OmcA molecules in the asymmetric unit are arranged so the minimum distance between heme 5 on adjacent OmcA monomers is 9 {\AA}, indicative of a transient OmcA dimer capable of intermolecular electron transfer. A previously identified hematite binding motif was identified near heme 10, forming a hydroxylated surface that would bring a heme 10 electron egress site to ∼10 {\AA} of a mineral surface.",

keywords = "Electron transfer, Metalloprotein, Mineral respiration, Multiheme cytochrome, Outer membrane, Shewanella, c-Type heme",

author = "Edwards, {Marcus J.} and Baiden, {Nanakow A.} and Alexander Johs and Tomanicek, {Stephen J.} and Liyuan Liang and Liang Shi and Fredrickson, {Jim K.} and Zachara, {John M.} and Gates, {Andrew J.} and Butt, {Julea N.} and Richardson, {David J.} and Clarke, {Thomas A.}",

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AU - Tomanicek, Stephen J.

AU - Liang, Liyuan

AU - Shi, Liang

AU - Fredrickson, Jim K.

AU - Zachara, John M.

AU - Gates, Andrew J.

AU - Butt, Julea N.

AU - Richardson, David J.

AU - Clarke, Thomas A.

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AB - The X-ray crystal structure of Shewanella oneidensis OmcA, an extracellular decaheme cytochrome involved in mineral reduction, was solved to a resolution of 2.7 Å. The four OmcA molecules in the asymmetric unit are arranged so the minimum distance between heme 5 on adjacent OmcA monomers is 9 Å, indicative of a transient OmcA dimer capable of intermolecular electron transfer. A previously identified hematite binding motif was identified near heme 10, forming a hydroxylated surface that would bring a heme 10 electron egress site to ∼10 Å of a mineral surface.

KW - Electron transfer

KW - Metalloprotein

KW - Mineral respiration

KW - Multiheme cytochrome

KW - Outer membrane

KW - Shewanella

KW - c-Type heme

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The X-ray crystal structure of Shewanella oneidensis OmcA reveals new insight at the microbe-mineral interface

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