Abstract
Development of microorganisms into mature bioproduction host strains has typically been a slow and circuitous process, wherein multiple groups apply disparate approaches with minimal coordination over decades. To help organize and streamline host development efforts, we introduce the Tier System for Host Development, a conceptual model and guide for developing microbial hosts that can ultimately lead to a systematic, standardized, less expensive, and more rapid workflow. The Tier System is made up of three Tiers, each consisting of a unique set of strain development Targets, including experimental tools, strain properties, experimental information, and process models. By introducing the Tier System, we hope to improve host development activities through standardization and systematization pertaining to nontraditional chassis organisms.
| Original language | English |
|---|---|
| Article number | 103260 |
| Journal | Current Opinion in Biotechnology |
| Volume | 92 |
| DOIs | |
| State | Published - Apr 2025 |
Funding
This work was part of the Agile BioFoundry (https://agilebiofoundry.org) supported by the U.S. Department of Energy, Office of Energy Efficiency and Renewable Energy, and Bioenergy Technologies Office under contract NL0032182. The work was authored under contracts between the U.S. Department of Energy and Lawrence Berkeley National Laboratory operated by the University of California (DE-AC02-05CH11231), Oak Ridge National Laboratory operated by UT-Battelle, LLC (DE-AC05-00OR22725), National Renewable Energy Laboratory operated by the Alliance for Sustainable Energy, LLC (DE-AC36-08GO28308), Pacific Northwest National Laboratory operated by Battelle (DE-AC05-76RLO1830), Argonne National Laboratory operated by UChicago Argonne, LLC (DE-AC02-06CH11357), and Los Alamos National Laboratory operated by Triad National Security, LLC (89233218CNA000001). The views and opinions of the authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof. Neither the United States Government nor any agency thereof, nor any of their employees, makes any warranty, expressed or implied, or assumes any legal liability or responsibility or the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed or represents that its use would not infringe privately owned rights. The United States Government retains, and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a nonexclusive, paid-up, irrevocable, worldwide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan). Funding for open access charge: US Department of Energy. We would like to thank Vivek Mutali for early conceptualization of the Tier System and Kyle Pomraning and Philip Labile for invaluable input and reviewing and editing the manuscript. This work was part of the Agile BioFoundry ( https://agilebiofoundry.org ) supported by the U.S. Department of Energy, Energy Efficiency and Renewable Energy, Bioenergy Technologies Office under contract NL0032182. The work was authored under contracts between the U.S. Department of Energy and Lawrence Berkeley National Laboratory operated by the University of California (DE-AC02-05CH11231), Oak Ridge National Laboratory operated by UT-Battelle, LLC (DE-AC05-00OR22725), National Renewable Energy Laboratory operated by the Alliance for Sustainable Energy, LLC (DE-AC36-08GO28308), Pacific Northwest National Laboratory operated by Battelle (DE-AC05-76RLO1830), Argonne National Laboratory operated by UChicago Argonne, LLC (DE-AC02-06CH11357), and Los Alamos National Laboratory operated by Triad National Security, LLC (89233218CNA000001).