The prokaryotic V4R domain is the likely ancestor of a key component of the eukaryotic vesicle transport system

Mircea Podar; Mark A. Wall; Kira S. Makarova; Eugene V. Koonin

doi:10.1186/1745-6150-3-2

The prokaryotic V4R domain is the likely ancestor of a key component of the eukaryotic vesicle transport system

Mircea Podar, Mark A. Wall, Kira S. Makarova, Eugene V. Koonin

Integrative Microbiomics

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Intracellular vesicle traffic that enables delivery of proteins between the endoplasmic reticulum, Golgi and various endosomal subcompartments is one of the hallmarks of the eukaryotic cell. Its evolutionary history is not well understood but the process itself and the core vesicle traffic machinery are believed to be ancient. We show here that the 4-vinyl reductase (V4R) protein domain present in bacteria and archaea is homologous to the Bet3 subunit of the TRAPP1 vesicle-tethering complex that is conserved in all eukaryotes. This suggests, for the first time, a prokaryotic origin for one of the key eukaryotic trafficking proteins.

Original language	English
Article number	2
Journal	Biology Direct
Volume	3
DOIs	https://doi.org/10.1186/1745-6150-3-2
State	Published - Jan 25 2008

Funding

MP is supported by the U.S. Department of Energy, Office of Science, Biological and Environmental Research programs at Oak Ridge National Laboratory (ORNL). ORNL is managed by UT-Battelle, LLC, for the U.S. Department of Energy under contract DE-AC05-00OR22725. KSM and EVK are supported by the Intramural Research Program of the National Institutes of Health, National Library of Medicine. M.P. acknowledges the support obtained from the Joint Genome Institute with the Ignicoccus hos-pitalis genome sequencing and analysis.

Funders	Funder number
National Institutes of Health	Z01LM000073
U.S. Department of Energy
U.S. National Library of Medicine
Oak Ridge National Laboratory	DE-AC05-00OR22725

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The prokaryotic V4R domain is the likely ancestor of a key component of the eukaryotic vesicle transport system

Abstract

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