TY - JOUR
T1 - The Impact of Acute Nutritional Interventions on the Plasma Proteome
AU - Vernardis, Spyros I.
AU - Demichev, Vadim
AU - Lemke, Oliver
AU - Grüning, Nana Maria
AU - Messner, Christoph
AU - White, Matt
AU - Pietzner, Maik
AU - Peluso, Alina
AU - Collet, Tinh Hai
AU - Henning, Elana
AU - Gille, Christoph
AU - Campbell, Archie
AU - Hayward, Caroline
AU - Porteous, David J.
AU - Marioni, Riccardo E.
AU - Mülleder, Michael
AU - Zelezniak, Aleksej
AU - Wareham, Nicholas J.
AU - Langenberg, Claudia
AU - Farooqi, I. Sadaf
AU - Ralser, Markus
N1 - Publisher Copyright:
© The Author(s) 2023. Published by Oxford University Press on behalf of the Endocrine Society.
PY - 2023/8/1
Y1 - 2023/8/1
N2 - Context: Humans respond profoundly to changes in diet, while nutrition and environment have a great impact on population health. It is therefore important to deeply characterize the human nutritional responses. Objective: Endocrine parameters and the metabolome of human plasma are rapidly responding to acute nutritional interventions such as caloric restriction or a glucose challenge. It is less well understood whether the plasma proteome would be equally dynamic, and whether it could be a source of corresponding biomarkers. Methods: We used high-throughput mass spectrometry to determine changes in the plasma proteome of i) 10 healthy, young, male individuals in response to 2 days of acute caloric restriction followed by refeeding; ii) 200 individuals of the Ely epidemiological study before and after a glucose tolerance test at 4 time points (0, 30, 60, 120 minutes); and iii) 200 random individuals from the Generation Scotland study. We compared the proteomic changes detected with metabolome data and endocrine parameters. Results: Both caloric restriction and the glucose challenge substantially impacted the plasma proteome. Proteins responded across individuals or in an individual-specific manner. We identified nutrient-responsive plasma proteins that correlate with changes in the metabolome, as well as with endocrine parameters. In particular, our study highlights the role of apolipoprotein C1 (APOC1), a small, understudied apolipoprotein that was affected by caloric restriction and dominated the response to glucose consumption and differed in abundance between individuals with and without type 2 diabetes. Conclusion: Our study identifies APOC1 as a dominant nutritional responder in humans and highlights the interdependency of acute nutritional response proteins and the endocrine system.
AB - Context: Humans respond profoundly to changes in diet, while nutrition and environment have a great impact on population health. It is therefore important to deeply characterize the human nutritional responses. Objective: Endocrine parameters and the metabolome of human plasma are rapidly responding to acute nutritional interventions such as caloric restriction or a glucose challenge. It is less well understood whether the plasma proteome would be equally dynamic, and whether it could be a source of corresponding biomarkers. Methods: We used high-throughput mass spectrometry to determine changes in the plasma proteome of i) 10 healthy, young, male individuals in response to 2 days of acute caloric restriction followed by refeeding; ii) 200 individuals of the Ely epidemiological study before and after a glucose tolerance test at 4 time points (0, 30, 60, 120 minutes); and iii) 200 random individuals from the Generation Scotland study. We compared the proteomic changes detected with metabolome data and endocrine parameters. Results: Both caloric restriction and the glucose challenge substantially impacted the plasma proteome. Proteins responded across individuals or in an individual-specific manner. We identified nutrient-responsive plasma proteins that correlate with changes in the metabolome, as well as with endocrine parameters. In particular, our study highlights the role of apolipoprotein C1 (APOC1), a small, understudied apolipoprotein that was affected by caloric restriction and dominated the response to glucose consumption and differed in abundance between individuals with and without type 2 diabetes. Conclusion: Our study identifies APOC1 as a dominant nutritional responder in humans and highlights the interdependency of acute nutritional response proteins and the endocrine system.
KW - APOC1
KW - caloric restriction
KW - oral glucose tolerance test
KW - plasma proteomics
KW - type 2 diabetes
UR - http://www.scopus.com/inward/record.url?scp=85161615336&partnerID=8YFLogxK
U2 - 10.1210/clinem/dgad031
DO - 10.1210/clinem/dgad031
M3 - Article
C2 - 36658456
AN - SCOPUS:85161615336
SN - 0021-972X
VL - 108
SP - 2087
EP - 2098
JO - The Journal of clinical endocrinology and metabolism
JF - The Journal of clinical endocrinology and metabolism
IS - 8
ER -