The histone demethylase KdmB is part of a trimeric protein complex and mediates virulence and mycotoxin production in Penicillium expansum

Dianiris Luciano-Rosario; Omer Barda; Joanna Tannous; Dean Frawley; Özgür Bayram; Dov Prusky; Edward Sionov; Nancy P. Keller

doi:10.1016/j.fgb.2023.103837

The histone demethylase KdmB is part of a trimeric protein complex and mediates virulence and mycotoxin production in Penicillium expansum

Dianiris Luciano-Rosario, Omer Barda, Joanna Tannous, Dean Frawley, Özgür Bayram, Dov Prusky, Edward Sionov, Nancy P. Keller

Synthetic Biology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Epigenetic modification of chromosome structure has increasingly been associated with alterations in secondary metabolism and sporulation defects in filamentous fungal pathogens. Recently, the epigenetic reader protein SntB was shown to govern virulence, spore production and mycotoxin synthesis in the fruit pathogen Penicillium expansum. Through immunoprecipitation-coupled mass spectrometry, we found that SntB is a member of a protein complex with KdmB, a histone demethylase and the essential protein RpdA, a histone deacetylase. Deletion of kdmB phenocopied some but not all characteristics of the ΔsntB mutant. KdmB deletion strains exhibited reduced lesion development on Golden Delicious apples and this was accompanied by decreased production of patulin and citrinin in host tissue. In addition, ΔkdmB mutants were sensitive to several cell wall stressors which possibly contributed to the decreased virulence observed on apples. Slight differences in spore production and germination rates of ΔkdmB mutants in vitro did not impact overall diameter growth in culture.

Original language	English
Article number	103837
Journal	Fungal Genetics and Biology
Volume	169
DOIs	https://doi.org/10.1016/j.fgb.2023.103837
State	Published - Dec 2023

Funding

This work was supported by Research Grant Award No. IS-5323-20C from BARD, the United States – Israel Binational Agricultural Research and Development Fund, to NPK and ES, by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under Award Number T32AI055397 and the University of Wisconsin-Madison Advanced Opportunity Fellowship, Science, and Medicine Graduate Research Scholars program to DL. ÖB was funded by Science Foundation Ireland, grant 21 FFP-P 10146. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health or BARD. DF was funded by an IRC postgraduate scholarship (GOIPG/2018/35).

Keywords

Epigenetics
Fungal biology
Mycotoxin
Post-harvest disease
Secondary metabolism
Virulence

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title = "The histone demethylase KdmB is part of a trimeric protein complex and mediates virulence and mycotoxin production in Penicillium expansum",

abstract = "Epigenetic modification of chromosome structure has increasingly been associated with alterations in secondary metabolism and sporulation defects in filamentous fungal pathogens. Recently, the epigenetic reader protein SntB was shown to govern virulence, spore production and mycotoxin synthesis in the fruit pathogen Penicillium expansum. Through immunoprecipitation-coupled mass spectrometry, we found that SntB is a member of a protein complex with KdmB, a histone demethylase and the essential protein RpdA, a histone deacetylase. Deletion of kdmB phenocopied some but not all characteristics of the ΔsntB mutant. KdmB deletion strains exhibited reduced lesion development on Golden Delicious apples and this was accompanied by decreased production of patulin and citrinin in host tissue. In addition, ΔkdmB mutants were sensitive to several cell wall stressors which possibly contributed to the decreased virulence observed on apples. Slight differences in spore production and germination rates of ΔkdmB mutants in vitro did not impact overall diameter growth in culture.",

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AU - Luciano-Rosario, Dianiris

AU - Barda, Omer

AU - Tannous, Joanna

AU - Frawley, Dean

AU - Bayram, Özgür

AU - Prusky, Dov

AU - Sionov, Edward

AU - Keller, Nancy P.

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N2 - Epigenetic modification of chromosome structure has increasingly been associated with alterations in secondary metabolism and sporulation defects in filamentous fungal pathogens. Recently, the epigenetic reader protein SntB was shown to govern virulence, spore production and mycotoxin synthesis in the fruit pathogen Penicillium expansum. Through immunoprecipitation-coupled mass spectrometry, we found that SntB is a member of a protein complex with KdmB, a histone demethylase and the essential protein RpdA, a histone deacetylase. Deletion of kdmB phenocopied some but not all characteristics of the ΔsntB mutant. KdmB deletion strains exhibited reduced lesion development on Golden Delicious apples and this was accompanied by decreased production of patulin and citrinin in host tissue. In addition, ΔkdmB mutants were sensitive to several cell wall stressors which possibly contributed to the decreased virulence observed on apples. Slight differences in spore production and germination rates of ΔkdmB mutants in vitro did not impact overall diameter growth in culture.

AB - Epigenetic modification of chromosome structure has increasingly been associated with alterations in secondary metabolism and sporulation defects in filamentous fungal pathogens. Recently, the epigenetic reader protein SntB was shown to govern virulence, spore production and mycotoxin synthesis in the fruit pathogen Penicillium expansum. Through immunoprecipitation-coupled mass spectrometry, we found that SntB is a member of a protein complex with KdmB, a histone demethylase and the essential protein RpdA, a histone deacetylase. Deletion of kdmB phenocopied some but not all characteristics of the ΔsntB mutant. KdmB deletion strains exhibited reduced lesion development on Golden Delicious apples and this was accompanied by decreased production of patulin and citrinin in host tissue. In addition, ΔkdmB mutants were sensitive to several cell wall stressors which possibly contributed to the decreased virulence observed on apples. Slight differences in spore production and germination rates of ΔkdmB mutants in vitro did not impact overall diameter growth in culture.

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KW - Mycotoxin

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The histone demethylase KdmB is part of a trimeric protein complex and mediates virulence and mycotoxin production in Penicillium expansum

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