The effect of deuteration on the structure of bacterial cellulose

Garima Bali; Marcus B. Foston; Hugh M. O'Neill; Barbara R. Evans; Junhong He; Arthur J. Ragauskas

doi:10.1016/j.carres.2013.04.009

The effect of deuteration on the structure of bacterial cellulose

Garima Bali, Marcus B. Foston, Hugh M. O'Neill, Barbara R. Evans, Junhong He, Arthur J. Ragauskas

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

In vivo generated deuterated bacterial cellulose, cultivated from 100% deuterated glycerol in D₂O medium, was analyzed for deuterium incorporation by ionic liquid dissolution and ²H and ¹H nuclear magnetic resonance (NMR). A solution NMR method of the dissolved cellulose was used to determine that this bacterial cellulose had 85% deuterium incorporation. Acetylation and ¹H and ²H NMR of deuterated bacterial cellulose indicated near equal deuteration at all sites of the glucopyranosyl ring except C-6 which was partly deuterated. Despite the high level of deuterium incorporation no significant differences in the molecular and morphological properties were observed for the deuterated and protio bacterial cellulose samples. The highly deuterated bacterial cellulose presented here can be used as a model substrate for studying cellulose biopolymer properties via future small angle neutron scattering (SANS) studies.

Original language	English
Pages (from-to)	82-88
Number of pages	7
Journal	Carbohydrate Research
Volume	374
DOIs	https://doi.org/10.1016/j.carres.2013.04.009
State	Published - Jun 7 2013

Funding

The support of the Center for Structural Molecular Biology funded by the U.S. DOE, Office of Science, Office of Biological and Environmental Research Project ERKP291 and the Scientific User Facilities Division, Basic Energy Sciences, U. S. DOE, are acknowledged for bio-deuteration studies.

Funders	Funder number
Office of Biological and Environmental Research Project	ERKP291
U.S. Department of Energy
Office of Science

Keywords

Acetobacter xylinus
Crystallinity
Deuterated bacterial cellulose
FTIR

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10.1016/j.carres.2013.04.009

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title = "The effect of deuteration on the structure of bacterial cellulose",

abstract = "In vivo generated deuterated bacterial cellulose, cultivated from 100% deuterated glycerol in D2O medium, was analyzed for deuterium incorporation by ionic liquid dissolution and 2H and 1H nuclear magnetic resonance (NMR). A solution NMR method of the dissolved cellulose was used to determine that this bacterial cellulose had 85% deuterium incorporation. Acetylation and 1H and 2H NMR of deuterated bacterial cellulose indicated near equal deuteration at all sites of the glucopyranosyl ring except C-6 which was partly deuterated. Despite the high level of deuterium incorporation no significant differences in the molecular and morphological properties were observed for the deuterated and protio bacterial cellulose samples. The highly deuterated bacterial cellulose presented here can be used as a model substrate for studying cellulose biopolymer properties via future small angle neutron scattering (SANS) studies.",

keywords = "Acetobacter xylinus, Crystallinity, Deuterated bacterial cellulose, FTIR",

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AU - Bali, Garima

AU - Foston, Marcus B.

AU - O'Neill, Hugh M.

AU - Evans, Barbara R.

AU - He, Junhong

AU - Ragauskas, Arthur J.

PY - 2013/6/7

Y1 - 2013/6/7

N2 - In vivo generated deuterated bacterial cellulose, cultivated from 100% deuterated glycerol in D2O medium, was analyzed for deuterium incorporation by ionic liquid dissolution and 2H and 1H nuclear magnetic resonance (NMR). A solution NMR method of the dissolved cellulose was used to determine that this bacterial cellulose had 85% deuterium incorporation. Acetylation and 1H and 2H NMR of deuterated bacterial cellulose indicated near equal deuteration at all sites of the glucopyranosyl ring except C-6 which was partly deuterated. Despite the high level of deuterium incorporation no significant differences in the molecular and morphological properties were observed for the deuterated and protio bacterial cellulose samples. The highly deuterated bacterial cellulose presented here can be used as a model substrate for studying cellulose biopolymer properties via future small angle neutron scattering (SANS) studies.

AB - In vivo generated deuterated bacterial cellulose, cultivated from 100% deuterated glycerol in D2O medium, was analyzed for deuterium incorporation by ionic liquid dissolution and 2H and 1H nuclear magnetic resonance (NMR). A solution NMR method of the dissolved cellulose was used to determine that this bacterial cellulose had 85% deuterium incorporation. Acetylation and 1H and 2H NMR of deuterated bacterial cellulose indicated near equal deuteration at all sites of the glucopyranosyl ring except C-6 which was partly deuterated. Despite the high level of deuterium incorporation no significant differences in the molecular and morphological properties were observed for the deuterated and protio bacterial cellulose samples. The highly deuterated bacterial cellulose presented here can be used as a model substrate for studying cellulose biopolymer properties via future small angle neutron scattering (SANS) studies.

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KW - Crystallinity

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KW - FTIR

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The effect of deuteration on the structure of bacterial cellulose

Abstract

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Keywords

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