Abstract
Most studies of sex determination systems in plants involve dioecious annuals that have known sex chromosomes. Despite the absence of such structures in the majority of dioecious plants, gender seems to be under relatively strict genetic control in some species. Genetic markers linked to a female sex-determination locus in Salix viminalis L. have been discovered through bulked segregant analysis of three full-sib families using approximately 1,000 arbitrary primers. Two RAPD markers that were present in the common female parent as well as in predominantly female progeny of these families were subsequently sequenced and converted to sequence characterized amplified region (SCAR) markers. The two SCAR markers are correlated with gender in the three full-sib families and are present in 96.4% of the female progeny and 2.2% of the males, providing evidence of linkage to a putative female-specific locus associated with gender determination in S. viminalis. Estimates of recombination suggest that the two markers are flanking a putative sex determination locus, SDL-II, in certain families of S. viminalis.
| Original language | English |
|---|---|
| Pages (from-to) | 185-189 |
| Number of pages | 5 |
| Journal | Journal of Heredity |
| Volume | 94 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 2003 |
Funding
From the Environmental Sciences Division, Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, TN 37831–6422 (Gunter, Roberts, Lee, and Tuskan) and the Life Sciences Division, Oak Ridge National Laboratory, P.O. Box 2008, Oak Ridge, TN (Larimer). This research was sponsored by the BioPower Program, Office of Power Technologies, Department of Energy Office of Energy Efficiency and Renewable Energy and performed at Oak Ridge National Laboratory (ORNL). ORNL is managed by UT-Battelle, LLC, for the U.S. Department of Energy under contract DE-AC05-00OR22725. We would like to thank the following: Dr. Cecilia Alström-Rapaport from the Department of Plant Breeding at the Swedish University of Agricultural Sciences, Uppsala, for providing us with plant material and for collaboration on modeling sex determination in S. viminalis; Dr. Richard Kopp at the State University of New York, College of Environmental Science and Forestry, Syracuse, NY, for providing DNA templates from other willow species for marker analysis; and both Dr. Kopp and Dr. Steve DiFazio for suggestions on the improvement of this manuscript.