The auxin efflux carrier PIN1a regulates vascular patterning in cereal roots

Riccardo Fusi, Sara Giulia Milner, Serena Rosignoli, Riccardo Bovina, Cristovão De Jesus Vieira Teixeira, Haoyu Lou, Brian S. Atkinson, Aditi N. Borkar, Larry M. York, Dylan H. Jones, Craig J. Sturrock, Nils Stein, Martin Mascher, Roberto Tuberosa, Devin O'Connor, Malcolm J. Bennett, Anthony Bishopp, Silvio Salvi, Rahul Bhosale

Research output: Contribution to journalArticlepeer-review

Abstract

Barley (Hordeum vulgare) is an important global cereal crop and a model in genetic studies. Despite advances in characterising barley genomic resources, few mutant studies have identified genes controlling root architecture and anatomy, which plays a critical role in capturing soil resources. Our phenotypic screening of a TILLING mutant collection identified line TM5992 exhibiting a short-root phenotype compared with wild-type (WT) Morex background. Outcrossing TM5992 with barley variety Proctor and subsequent SNP array-based bulk segregant analysis, fine mapped the mutation to a cM scale. Exome sequencing pinpointed a mutation in the candidate gene HvPIN1a, further confirming this by analysing independent mutant alleles. Detailed analysis of root growth and anatomy in Hvpin1a mutant alleles exhibited a slower growth rate, shorter apical meristem and striking vascular patterning defects compared to WT. Expression and mutant analyses of PIN1 members in the closely related cereal brachypodium (Brachypodium distachyon) revealed that BdPIN1a and BdPIN1b were redundantly expressed in root vascular tissues but only Bdpin1a mutant allele displayed root vascular defects similar to Hvpin1a. We conclude that barley PIN1 genes have sub-functionalised in cereals, compared to Arabidopsis (Arabidopsis thaliana), where PIN1a sequences control root vascular patterning.

Original languageEnglish
Pages (from-to)104-115
Number of pages12
JournalNew Phytologist
Volume244
Issue number1
DOIs
StatePublished - Oct 2024

Funding

We acknowledge the technical support of Simona Corneti, Anne Fiebig, Sandra Stefanelli. RF acknowledges the Hounsfield Facility Center at the University of Nottingham for all the support provided for this work. The Hounsfield Facility received funding from European Research Council (Futureroots Project, Grant agreement ID: 294729), Biotechnology and Biological Sciences Research Council of the United Kingdom and The Wolfson Foundation. HL was supported by a joint University of Adelaide\u2009\u2013\u2009University of Nottingham PhD scholarship. This manuscript has been authored in part by LMY at UT\u2010Battelle, LLC, under contract DE\u2010AC05\u201000OR22725 with the US Department of Energy (DOE). The publisher acknowledges the US government licence to provide public access under the DOE Public Access Plan ( http://energy.gov/downloads/doe-public-access\u2010plan ). R. Bhosale acknowledges support from BBSRC Discovery Fellowship (BB/S011102/1), BBSRC New Investigator grant (BB/X014843/1), and BBSRC\u2010funded Delivering Sustainable Wheat (DSW) Partner Grant (BB/X018806/1).

FundersFunder number
Biotechnology and Biological Sciences Research Council of the United Kingdom and The Wolfson Foundation
BBSRC‐funded Delivering Sustainable Wheat
U.S. Department of Energy
Hounsfield Facility Center at the University of Nottingham
joint University of Adelaide – University of NottinghamDE‐AC05‐00OR22725
European Research Council294729
European Research Council
Biotechnology and Biological Sciences Research CouncilBB/S011102/1, BB/X014843/1
Biotechnology and Biological Sciences Research Council
DSWBB/X018806/1

    Keywords

    • PIN1
    • auxin efflux carrier
    • barley
    • root
    • vascular development

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