TEMPORARY REMOVAL: The antioxidant vitamin E as a membrane raft modulator: Tocopherols do not abolish lipid domains

Mitchell DiPasquale; Michael H.L. Nguyen; Brett W. Rickeard; Nicole Cesca; Christopher Tannous; Stuart R. Castillo; John Katsaras; Elizabeth G. Kelley; Frederick A. Heberle; Drew Marquardt

doi:10.1016/j.bbamem.2020.183189

TEMPORARY REMOVAL: The antioxidant vitamin E as a membrane raft modulator: Tocopherols do not abolish lipid domains

Mitchell DiPasquale, Michael H.L. Nguyen, Brett W. Rickeard, Nicole Cesca, Christopher Tannous, Stuart R. Castillo, John Katsaras, Elizabeth G. Kelley, Frederick A. Heberle, Drew Marquardt

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Abstract

The antioxidant vitamin E is a commonly used vitamin supplement. Although the multi-billion dollar vitamin and nutritional supplement industry encourages the use of vitamin E, there is very little evidence supporting its actual health benefits. Moreover, vitamin E is now marketed as a lipid raft destabilizing anti-cancer agent, in addition to its antioxidant behaviour. Here, we studied the influence of vitamin E and some of its vitamers on membrane raft stability using phase separating unilamellar lipid vesicles in conjunction with small-angle scattering techniques and fluorescence microscopy. We find that lipid phase behaviour remains unperturbed well beyond physiological concentrations of vitamin E (up to a mole fraction of 0.10). Our results are consistent with a proposed line active role of vitamin E at the domain boundary. We discuss the implications of these findings as they pertain to lipid raft modification in native membranes, and propose a new hypothesis for the antioxidant mechanism of vitamin E.

Original language	English
Article number	183189
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1862
Issue number	8
DOIs	https://doi.org/10.1016/j.bbamem.2020.183189
State	Published - Aug 1 2020

Funding

We acknowledge the support of the National Institute of Standards and Technology, U.S. Department of Commerce in providing access to the NG3 VSANS instrument through the Center for High Resolution Neutron Scattering, a partnership between the National Science Foundation and the National Institute of Standards and Technology under Agreement DMR-1508249. Certain commercial equipment or materials are identified in the paper to foster understanding. Such identification does not imply recommendation or endorsement by the National Institute of Standards and Technology, nor does it imply that the materials or equipment identified are necessarily the best available for the purpose. A portion of this research used resources from the CG3 BioSANS instrument at the High Flux Isotope Reactor, a DOE Office of Science User Facility operated by the Oak Ridge National Laboratory. J.K is supported through the Scientific User Facilities Division of the Department of Energy Office of Science, sponsored by the Basic Energy Science Program, Department of Energy Office of Science, under contract number DEAC05-00OR22725. The authors thank Shuo Qian for his BioSANS technical assistance and Georg Pabst for access to the GAP program. F.A.H acknowledges support from National Science Foundation grant No. MCB-1817929. M.D., M.H.L.N. and B.W.R. are supported by Ontario Graduate Scholarships. D.M. acknowledges the support of the Natural Sciences and Engineering Research Council of Canada (NSERC), [funding reference number RGPIN-2018-04841] and the University of Windsor start-up funds.

Funders	Funder number
U.S. Department of Commerce
University of Windsor
National Institute of Standards and Technology
Office of Science
Basic Energy Science Program	MCB-1817929, DEAC05-00OR22725
National Science Foundation	DMR-1508249, 1817929
Natural Sciences and Engineering Research Council of Canada	unidentified, RGPIN-2018-04841

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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DiPasquale, M., Nguyen, M. H. L., Rickeard, B. W., Cesca, N., Tannous, C., Castillo, S. R., Katsaras, J., Kelley, E. G., Heberle, F. A., & Marquardt, D. (2020). TEMPORARY REMOVAL: The antioxidant vitamin E as a membrane raft modulator: Tocopherols do not abolish lipid domains. Biochimica et Biophysica Acta - Biomembranes, 1862(8), Article 183189. https://doi.org/10.1016/j.bbamem.2020.183189

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abstract = "The antioxidant vitamin E is a commonly used vitamin supplement. Although the multi-billion dollar vitamin and nutritional supplement industry encourages the use of vitamin E, there is very little evidence supporting its actual health benefits. Moreover, vitamin E is now marketed as a lipid raft destabilizing anti-cancer agent, in addition to its antioxidant behaviour. Here, we studied the influence of vitamin E and some of its vitamers on membrane raft stability using phase separating unilamellar lipid vesicles in conjunction with small-angle scattering techniques and fluorescence microscopy. We find that lipid phase behaviour remains unperturbed well beyond physiological concentrations of vitamin E (up to a mole fraction of 0.10). Our results are consistent with a proposed line active role of vitamin E at the domain boundary. We discuss the implications of these findings as they pertain to lipid raft modification in native membranes, and propose a new hypothesis for the antioxidant mechanism of vitamin E.",

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DiPasquale, M, Nguyen, MHL, Rickeard, BW, Cesca, N, Tannous, C, Castillo, SR, Katsaras, J, Kelley, EG, Heberle, FA & Marquardt, D 2020, 'TEMPORARY REMOVAL: The antioxidant vitamin E as a membrane raft modulator: Tocopherols do not abolish lipid domains', Biochimica et Biophysica Acta - Biomembranes, vol. 1862, no. 8, 183189. https://doi.org/10.1016/j.bbamem.2020.183189

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AU - Tannous, Christopher

AU - Castillo, Stuart R.

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AU - Marquardt, Drew

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TEMPORARY REMOVAL: The antioxidant vitamin E as a membrane raft modulator: Tocopherols do not abolish lipid domains

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