Temporal dynamics of protein and post-translational modification abundances in Populus leaf across a diurnal period

Him K. Shrestha; Manuel I.Villalobos Solis; Sara S. Jawdy; Gerald A. Tuskan; Xiaohan Yang; Paul E. Abraham

doi:10.1002/pmic.202100127

Temporal dynamics of protein and post-translational modification abundances in Populus leaf across a diurnal period

Him K. Shrestha, Manuel I.Villalobos Solis, Sara S. Jawdy, Gerald A. Tuskan, Xiaohan Yang, Paul E. Abraham

Research output: Contribution to journal › Article › peer-review

Abstract

Populus spp. are dedicated woody biomass feedstocks for advanced biofuels and bioproducts. Proper growth and fitness of poplar as a sustainable feedstock depends on timely perception and response to environmental signals (e.g., light, temperature, water). Poplar leaves, like other C3 photosynthesis plants, have evolved oscillating or circadian rhythms that play important roles in synchronizing biological processes with external cues. To characterize this phenomenon at a molecular level, we employed bottom-up proteomics using high-resolution mass spectrometry and de novo-assisted database searching to identify abundance changes in proteins and post-translational modifications in poplar leaf tissue sampled across a 12/12-hour light/dark diurnal period.

Original language	English
Article number	2100127
Journal	Proteomics
Volume	21
Issue number	20
DOIs	https://doi.org/10.1002/pmic.202100127
State	Published - Oct 2021

Funding

Notice: This manuscript has been authored by UT‐Battelle, LLC under Contract No. DE‐AC05‐ 00OR22725 with the U.S. Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non‐exclusive, paid‐up, irrevocable, worldwide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan ( http://energy.gov/downloads/doe‐public‐access‐plan ). This research was sponsored by the Genomic Science Program, U.S. Department of Energy, Office of Science, Biological and Environmental Research, as part of the Center for Bioenergy Innovation (CBI) ( https://cbi.ornl.gov/ ). Oak Ridge National Laboratory is managed by UT‐Battelle, LLC, for the U.S. Department of Energy under contract DE‐AC05‐00OR22725. This research was sponsored by the Genomic Science Program, U.S. Department of Energy, Office of Science, Biological and Environmental Research, as part of the Center for Bioenergy Innovation (CBI) (https://cbi.ornl.gov/). Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the U.S. Department of Energy under contract DE-AC05-00OR22725.

Funders	Funder number
U.S. Department of Energy
Office of Science
Biological and Environmental Research
Oak Ridge National Laboratory	DE‐AC05‐ 00OR22725
Center for Bioenergy Innovation	DE‐AC05‐00OR22725

Keywords

Populus
diurnal rhythm
post-translational modifications
proteomics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/pmic.202100127

Cite this

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title = "Temporal dynamics of protein and post-translational modification abundances in Populus leaf across a diurnal period",

abstract = "Populus spp. are dedicated woody biomass feedstocks for advanced biofuels and bioproducts. Proper growth and fitness of poplar as a sustainable feedstock depends on timely perception and response to environmental signals (e.g., light, temperature, water). Poplar leaves, like other C3 photosynthesis plants, have evolved oscillating or circadian rhythms that play important roles in synchronizing biological processes with external cues. To characterize this phenomenon at a molecular level, we employed bottom-up proteomics using high-resolution mass spectrometry and de novo-assisted database searching to identify abundance changes in proteins and post-translational modifications in poplar leaf tissue sampled across a 12/12-hour light/dark diurnal period.",

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author = "Shrestha, {Him K.} and Solis, {Manuel I.Villalobos} and Jawdy, {Sara S.} and Tuskan, {Gerald A.} and Xiaohan Yang and Abraham, {Paul E.}",

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AU - Solis, Manuel I.Villalobos

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AU - Yang, Xiaohan

AU - Abraham, Paul E.

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AB - Populus spp. are dedicated woody biomass feedstocks for advanced biofuels and bioproducts. Proper growth and fitness of poplar as a sustainable feedstock depends on timely perception and response to environmental signals (e.g., light, temperature, water). Poplar leaves, like other C3 photosynthesis plants, have evolved oscillating or circadian rhythms that play important roles in synchronizing biological processes with external cues. To characterize this phenomenon at a molecular level, we employed bottom-up proteomics using high-resolution mass spectrometry and de novo-assisted database searching to identify abundance changes in proteins and post-translational modifications in poplar leaf tissue sampled across a 12/12-hour light/dark diurnal period.

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Temporal dynamics of protein and post-translational modification abundances in Populus leaf across a diurnal period

Abstract

Funding

Keywords

UN SDGs

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