@inproceedings{d0ffee8375ef4d8fa590b2dd95042ee2,
title = "Temperature-controlled separations for improving the sensitivity of multi-color microfluidic immunoassays",
abstract = "A microfluidic device with integrated temperature control for the electrophoretic separation and conduction of immunoassays is introduced. The separation channel is actively cooled with the use of a thermoelectric cooler (TEC) embedded into the microfluidic housing manifold. The introduction of the cooling also allowed for the utilization of shorter separation distances with higher separation voltages with effective dissipation of Joule heating. The optimization effects were evaluated with a kinetic CE method known as non-equilibrium capillary electrophoresis of non-equilibrium mixture (NECEEM). This method of active cooling will be applicable to other microfluidic affinity separation systems.",
keywords = "Affinity assay, Kinetic capillary electrophoresis, Temperature effects",
author = "N. Mukhitov and L. Yi and Schrell, {A. M.} and X. Wang and R. Dhumpa and Roper, {M. G.}",
note = "Publisher Copyright: {\textcopyright} 14CBMS.; 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",
year = "2014",
language = "English",
series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
publisher = "Chemical and Biological Microsystems Society",
pages = "2429--2431",
booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
}