Telomere repeats induce domains of H3K27 methylation in Neurospora

Kirsty Jamieson; Kevin J. McNaught; Tereza Ormsby; Neena A. Leggett; Shinji Honda; Eric U. Selker

doi:10.7554/eLife.31216

Telomere repeats induce domains of H3K27 methylation in Neurospora

Kirsty Jamieson, Kevin J. McNaught, Tereza Ormsby, Neena A. Leggett, Shinji Honda, Eric U. Selker

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Development in higher organisms requires selective gene silencing, directed in part by di-/trimethylation of lysine 27 on histone H3 (H3K27me2/3). Knowledge of the cues that control formation of such repressive Polycomb domains is extremely limited. We exploited natural and engineered chromosomal rearrangements in the fungus Neurospora crassa to elucidate the control of H3K27me2/3. Analyses of H3K27me2/3 in strains bearing chromosomal rearrangements revealed both position-dependent and position-independent facultative heterochromatin. We found that proximity to chromosome ends is necessary to maintain, and sufficient to induce, transcriptionally repressive, subtelomeric H3K27me2/3. We ascertained that such telomere-proximal facultative heterochromatin requires native telomere repeats and found that a short array of ectopic telomere repeats, (TTAGGG)₁₇, can induce a large domain (~225 kb) of H3K27me2/3. This provides an example of a cis-acting sequence that directs H3K27 methylation. Our findings provide new insight into the relationship between genome organization and control of heterochromatin formation.

Original language	English
Article number	e31216
Journal	eLife
Volume	7
DOIs	https://doi.org/10.7554/eLife.31216
State	Published - Jan 3 2018
Externally published	Yes

Funding

We thank Heejeung Yoo for genotyping csr-1 targeting strains. This study was supported by a National Institutes of Health grant (GM093061) to EUS and a grant to SH from the Japanese Program to Disseminate Tenure Tracking System, Ministry of Education, Culture, Sports, Science and Technology.

Funders	Funder number
National Institutes of Health	GM093061
National Institutes of Health
National Institute of Child Health and Human Development	T32HD007348
National Institute of Child Health and Human Development
Ministry of Education, Culture, Sports, Science and Technology

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title = "Telomere repeats induce domains of H3K27 methylation in Neurospora",

abstract = "Development in higher organisms requires selective gene silencing, directed in part by di-/trimethylation of lysine 27 on histone H3 (H3K27me2/3). Knowledge of the cues that control formation of such repressive Polycomb domains is extremely limited. We exploited natural and engineered chromosomal rearrangements in the fungus Neurospora crassa to elucidate the control of H3K27me2/3. Analyses of H3K27me2/3 in strains bearing chromosomal rearrangements revealed both position-dependent and position-independent facultative heterochromatin. We found that proximity to chromosome ends is necessary to maintain, and sufficient to induce, transcriptionally repressive, subtelomeric H3K27me2/3. We ascertained that such telomere-proximal facultative heterochromatin requires native telomere repeats and found that a short array of ectopic telomere repeats, (TTAGGG)17, can induce a large domain (~225 kb) of H3K27me2/3. This provides an example of a cis-acting sequence that directs H3K27 methylation. Our findings provide new insight into the relationship between genome organization and control of heterochromatin formation.",

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AU - McNaught, Kevin J.

AU - Ormsby, Tereza

AU - Leggett, Neena A.

AU - Honda, Shinji

AU - Selker, Eric U.

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Y1 - 2018/1/3

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Telomere repeats induce domains of H3K27 methylation in Neurospora

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