Abstract
Noninvasive investigation of cellular and molecular processes becomes possible through the novel techniques, one of which is molecular imaging, where enhanced sensitivity is a key component for clinic translation of the technique. In this presentation, spontaneously forming, small unilamellar vesicles (ULVs) (30 nm in diameter) were used as a platform to build a bi-modal [i.e., optical and Magnetic Resonance Imaging (MRI)] targeted contrast agent for the molecular imaging of brain tumors. Small ULVs were loaded with a gadolinium (Gd) chelated lipid (Gd-DPTA-BOA), functionalized with targeting antibodies (anti-EGFR monoclonal and anti-IGFBP7 single domain), and incorporated a near infrared dye (Cy5.5). The resultant ULVs were characterized in vitro using small angle neutron scattering (SANS), in phantom MRI and dynamic light scattering (DLS). Targeted (with antibodies) and nontargeted-Gd loaded sULVs labeled with Cy5.5 were assessed in vivo in a mice brain tumor model using both optical imaging and MRI. The results demonstrated that a spontaneously forming, nanosized ULV loaded with a high payload of Gd can selectively target and image, using MR and optical imaging, brain tumor vessels when functionalized with antibodies. The unique features of these targeting ULVs make them promising molecular MRI contrast agents.
| Original language | English |
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| Title of host publication | Nanoscale Science and Engineering Forum - Core Programming Topic at the 2011 AIChE Annual Meeting |
| Publisher | AIChE |
| Pages | 245-253 |
| Number of pages | 9 |
| ISBN (Print) | 9781618397416 |
| State | Published - 2011 |
| Event | Nanoscale Science and Engineering Forum - Core Programming Topic at the 2011 AIChE Annual Meeting - Minneapolis, United States Duration: Oct 16 2011 → Oct 21 2011 |
Publication series
| Name | Nanoscale Science and Engineering Forum - Core Programming Topic at the 2011 AIChE Annual Meeting |
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Conference
| Conference | Nanoscale Science and Engineering Forum - Core Programming Topic at the 2011 AIChE Annual Meeting |
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| Country/Territory | United States |
| City | Minneapolis |
| Period | 10/16/11 → 10/21/11 |
Funding
The authors acknowledge P Forsyth, X Lun and D Rushforth's contribution to producing the mice tumor model, and T Foniok and D Kirk for their assistance with the acquisition of MR images using these mice. We would also like to thank D Fatehi for her assistance with cell culture experiments and T Devesceri for his help with graphic and image processing. The authors acknowledge the support of NIST NCNR in providing the neutron research facilities used in this work. The SANS work made use of facilities supported in part by the National Science Foundation under Agreement No. DMR-0944772. MPN would like to thank for the financial support from the UConn Faculty Large Grant and the startup fund from the institute of materials science (IMS) at UConn. mice tumor model, and T Foniok and D Kirk for their assistance with the acquisition of MR images using these mice. We would also like to thank D Fatehi for her assistance with cell culture experiments and T Devesceri for his help with graphic and image processing. The authors acknowledge the support of NIST NCNR in providing the neutron research facilities used in this work. The SANS work made use of facilities supported in part by the National Science Foundation under Agreement No. DMR-0944772. MPN would like to thank for the financial support from the UConn Faculty Large Grant and the startup fund from the institute of materials science (IMS) at UConn.