Surface Modification of Silicon Pillar Arrays to Enhance Fluorescence Detection of Uranium and DNA

Danielle R. Lincoln; Jennifer J. Charlton; Nahla A. Hatab; Brittany Skyberg; Nickolay V. Lavrik; Ivan I. Kravchenko; James A. Bradshaw; Michael J. Sepaniak

doi:10.1021/acsomega.7b00912

Surface Modification of Silicon Pillar Arrays to Enhance Fluorescence Detection of Uranium and DNA

Danielle R. Lincoln, Jennifer J. Charlton, Nahla A. Hatab, Brittany Skyberg, Nickolay V. Lavrik, Ivan I. Kravchenko, James A. Bradshaw, Michael J. Sepaniak

Nanofabrication Research Laboratory

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

There is an ever-growing need for detection methods that are both sensitive and efficient, such that reagent and sample consumption is minimized. Nanopillar arrays offer an attractive option to fill this need by virtue of their small scale in conjunction with their field enhancement intensity gains. This work investigates the use of nanopillar substrates for the detection of the uranyl ion and DNA, two analytes unalike but for their low quantum efficiencies combined with the need for high-throughput analyses. Herein, the adaptability of these platforms was explored, as methods for the successful surface immobilization of both analytes were developed and compared, resulting in a limit of detection for the uranyl ion of less than 1 ppm with a 0.2 μL sample volume. Moreover, differentiation between single-stranded and double-stranded DNA was possible, including qualitative identification between double-stranded DNA and DNA of the same sequence, but with a 10-base-pair mismatch.

Original language	English
Pages (from-to)	7313-7319
Number of pages	7
Journal	ACS Omega
Volume	2
Issue number	10
DOIs	https://doi.org/10.1021/acsomega.7b00912
State	Published - Oct 31 2017

Funding

This material is based upon work supported by the National Science Foundation under grant no. 1144947 with the University of Tennessee, Knoxville. A portion of this research was conducted at the Center for Nanophase Materials Sciences, which is a DOE Office of Science User Facility, as well at the Y-12 National Security Complex. We also acknowledge John R. Dunlap, Ph.D., and the JIAM Microscopy Center and Advanced Microscopy and Imaging Center at UTK for access to facilities.

Funders	Funder number
National Science Foundation
Directorate for Mathematical and Physical Sciences	1144947
University of Tennessee

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title = "Surface Modification of Silicon Pillar Arrays to Enhance Fluorescence Detection of Uranium and DNA",

abstract = "There is an ever-growing need for detection methods that are both sensitive and efficient, such that reagent and sample consumption is minimized. Nanopillar arrays offer an attractive option to fill this need by virtue of their small scale in conjunction with their field enhancement intensity gains. This work investigates the use of nanopillar substrates for the detection of the uranyl ion and DNA, two analytes unalike but for their low quantum efficiencies combined with the need for high-throughput analyses. Herein, the adaptability of these platforms was explored, as methods for the successful surface immobilization of both analytes were developed and compared, resulting in a limit of detection for the uranyl ion of less than 1 ppm with a 0.2 μL sample volume. Moreover, differentiation between single-stranded and double-stranded DNA was possible, including qualitative identification between double-stranded DNA and DNA of the same sequence, but with a 10-base-pair mismatch.",

author = "Lincoln, {Danielle R.} and Charlton, {Jennifer J.} and Hatab, {Nahla A.} and Brittany Skyberg and Lavrik, {Nickolay V.} and Kravchenko, {Ivan I.} and Bradshaw, {James A.} and Sepaniak, {Michael J.}",

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AU - Lincoln, Danielle R.

AU - Charlton, Jennifer J.

AU - Hatab, Nahla A.

AU - Skyberg, Brittany

AU - Lavrik, Nickolay V.

AU - Kravchenko, Ivan I.

AU - Bradshaw, James A.

AU - Sepaniak, Michael J.

PY - 2017/10/31

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AB - There is an ever-growing need for detection methods that are both sensitive and efficient, such that reagent and sample consumption is minimized. Nanopillar arrays offer an attractive option to fill this need by virtue of their small scale in conjunction with their field enhancement intensity gains. This work investigates the use of nanopillar substrates for the detection of the uranyl ion and DNA, two analytes unalike but for their low quantum efficiencies combined with the need for high-throughput analyses. Herein, the adaptability of these platforms was explored, as methods for the successful surface immobilization of both analytes were developed and compared, resulting in a limit of detection for the uranyl ion of less than 1 ppm with a 0.2 μL sample volume. Moreover, differentiation between single-stranded and double-stranded DNA was possible, including qualitative identification between double-stranded DNA and DNA of the same sequence, but with a 10-base-pair mismatch.

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Surface Modification of Silicon Pillar Arrays to Enhance Fluorescence Detection of Uranium and DNA

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