31P NMR Characterization of Tricin and Its Structurally Similar Flavonoids

Mi Li, Yunqiao Pu, Timothy J. Tschaplinski, Arthur J. Ragauskas

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Abstract: Tricin, a flavonoid metabolite, has been recently identified as a component of lignin in select monocot plants. This finding has initiated consideration on updating the lignin biosynthesis pathway. We here report a rapid method of determination of tricin in corn stover lignin, based on 31P nuclear magnetic resonance (NMR) spectroscopy by phosphitylating with 2-chloro-4,4,5,5-tetramethyl-1,3,2-dioxaphospholane (TMDP). Nine other flavonoids, with similar structure to tricin, have also been examined using the current method. The application of 31P NMR enables rapid identification of tricin-like flavonoids in the heterogeneous lignin polymer. The well resolved spectroscopic peaks from these derivatized flavonoids and lignin functional groups provide important information for the determination of flavonoids individually or their association with lignin.

Original languageEnglish
Pages (from-to)3557-3561
Number of pages5
JournalChemistrySelect
Volume2
Issue number12
DOIs
StatePublished - Apr 24 2017

Funding

Oak Ridge National Laboratory (ORNL) is managed by UT-Battelle, LLC under Contract No. DE-AC05-00OR22725 with the U.S. Department of Energy (DOE). This study was supported and performed as part of the BioEnergy Science Center (BESC). The BESC is a U.S. DOE Bioenergy Research Center supported by the Office of

Keywords

  • NMR spectroscopy
  • biomass
  • flavonoids
  • lignin
  • tricin

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