Succinic acid adsorption from fermentation broth and regeneration

Brian H. Davison, Nhuan P. Nghiem, Gerald L. Richardson

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

More than 25 sorbents were tested for uptake of succinic acid from aqueous solutions. The best resins were then tested for successive loading and regeneration using hot water. The key desired properties for an ideal sorbent are high capacity, complete stable regenerability, and specificity for the product. The best resins have a stable capacity of about 0.06 g of succinic acid/g of resin at moderate concentrations (1-5 g/L) of succinic acid. Several sorbents were tested more exhaustively for uptake of succinic acid and for successive loading and regeneration using hot water. One resin, XUS 40285, has a good stable isotherm capacity, prefers succinate over glucose, and has good capacities at both acidic and neutral pH. Succinic acid was removed from simulated media containing salts, succinic acid, acetic acid, and sugar using a packed column of sorbent resin, XUS 40285. The fermentation byproduct, acetate, was completely separated from succinate. A simple hot water regeneration successfully concentrated succinate from 10 g/L (inlet) to 40-110 g/L in the effluent. If successful, this would lower separation costs by reducing the need for chemicals for the initial purification step. Despite promising initial results of good capacity (0.06 g of succinic/g of sorbent), 70% recovery using hot water, and a recovered concentration of >100 g/L, this regeneration was not stable over 10 cycles in the column. Alternative regeneration schemes using acid and base were examined. Two (XUS 40285 and XFS-40422) showed both good stable capacities for succinic acid over 10 cycles and >95% recovery in a batch operation using a modified extraction procedure combining acid and hot water washes. These resins showed comparable results with actual broth.

Original languageEnglish
Pages (from-to)653-669
Number of pages17
JournalApplied Biochemistry and Biotechnology
Volume114
Issue number1-3
DOIs
StatePublished - 2004

Funding

Funding was provided by the US Department of Energy—EERE— Office of Industrial Technologies. ORNL is operated by UT Battelle under contract with the US Department of Energy. Actual succinic acid fermentation broth was kindly provided by Michigan Biotechnology Institute.

FundersFunder number
Office of Industrial Technologies
US Department of Energy
Battelle
Office of Energy Efficiency and Renewable Energy

    Keywords

    • Adsorption
    • Fermentation broth
    • Glucose
    • Hot water regeneration
    • Sorbent
    • Succinic acid

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