Abstract
The interaction between catalytically active and inactive Trichoderma reesei cellulase components and cotton fibers has been examined by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Cellobiohydrolase I (CBH I), the major component, was rendered catalytically inactive by its treatment with ammonium hexachloropalladate; however, the inactive enzyme still had the ability to bind to the cotton fiber. SEM and AFM provided evidence suggesting that the catalytic activity of CBH I was required for fiber disruption AFM allowing resolution of cotton fibers to the microfibril level. However at high magnification slightly elongated holes were observed throughout the surface of the microfibrillar surface of cotton fibers treated with inactivated CBH I. No disruption of cotton fibers was observed by a palladium-inactivated CBH II/EG II mixture.
| Original language | English |
|---|---|
| Pages (from-to) | 163-169 |
| Number of pages | 7 |
| Journal | Bioresource Technology |
| Volume | 58 |
| Issue number | 2 |
| DOIs | |
| State | Published - Nov 1996 |
Funding
The authorst hank Mark Reevesf or reviewingt he manuscriptM, ary Jo Kreger for secretariasle rvices, and Marsha Savagef or editingt he manuscriptT.h is work was supportedb y the Division of Chemical Sciences, Office of Basic Energy Sciences, U.S. Departmenotf Energy,O ak Ridge NationalL abora-tory is managed by Lockheed Martin Energy Systems,In c., for the U.S. Departmenot f Energy underc ontracDt E-AC05-84OR21400.
| Funders | Funder number |
|---|---|
| Division of Chemical Sciences | |
| U.S. Departmenot f Energy | E-AC05-84OR21400 |
| U.S. Departmenotf Energy | |
| Lockheed Martin | |
| Basic Energy Sciences |
Keywords
- Atomic force microscopy
- Cellobiohydrolase I
- Cellulase
- Cotton fiber disruption
- Palladium inactivation