TY - JOUR
T1 - Structure of Chlorobium vibrioforme 5-aminolaevulinic acid dehydratase complexed with a diacid inhibitor
AU - Coates, L.
AU - Beaven, G.
AU - Erskine, P. T.
AU - Beale, Samuel I.
AU - Wood, S. P.
AU - Shoolingin-Jordan, P. M.
AU - Cooper, J. B.
PY - 2005/12
Y1 - 2005/12
N2 - The structure of Chlorobium vibrioforme 5-aminolaevulinic acid dehydratase (ALAD) complexed with the irreversible inhibitor 4,7-dioxosebacic acid has been solved. The inhibitor binds by forming Schiff-base linkages with lysines 200 and 253 at the active site. The structure reported here provides a definition of the interactions made by both of the substrate molecules (A-side and P-side substrates) with the C. vibrioforme ALAD and is compared and contrasted with structures of the same inhibitor bound to Escherichia coli and yeast ALAD. The structure suggests why 4,7-dioxosebacic acid is a better inhibitor of the zinc-dependent ALADs than of the zinc-independent ALADs.
AB - The structure of Chlorobium vibrioforme 5-aminolaevulinic acid dehydratase (ALAD) complexed with the irreversible inhibitor 4,7-dioxosebacic acid has been solved. The inhibitor binds by forming Schiff-base linkages with lysines 200 and 253 at the active site. The structure reported here provides a definition of the interactions made by both of the substrate molecules (A-side and P-side substrates) with the C. vibrioforme ALAD and is compared and contrasted with structures of the same inhibitor bound to Escherichia coli and yeast ALAD. The structure suggests why 4,7-dioxosebacic acid is a better inhibitor of the zinc-dependent ALADs than of the zinc-independent ALADs.
UR - http://www.scopus.com/inward/record.url?scp=33644836953&partnerID=8YFLogxK
U2 - 10.1107/S0907444905030350
DO - 10.1107/S0907444905030350
M3 - Article
C2 - 16304458
AN - SCOPUS:33644836953
SN - 0907-4449
VL - 61
SP - 1594
EP - 1598
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 12
ER -