Abstract
The rhizosphere is the narrow region of soil surrounding the roots of plants that is influenced by root exudates, root secretions, and associated microbial communities. This region is crucial to plant growth and development and plays a critical role in nutrient uptake, disease resistance, and soil transformation. Understanding the function of exogenous compounds in the rhizosphere starts with determining the spatiotemporal distribution of these molecular components. Using liquid microjunction surface-sampling probe mass spectrometry (LMJ-SSP-MS) and microfluidic devices with attached microporous membranes enables in situ, nondisruptive, and nondestructive spatiotemporal measurement of exogenous compounds from plant roots. However, long imaging times (>2 h) can negatively affect plant heath and limit temporal studies. Here, we present a novel strategy to optimize the number and location of sampling sites on these microporous membrane-covered microfluidic devices. This novel, “structure-driven” sampling workflow takes into consideration the channel structure of the microfluidic device to maximize sampling from the channels and minimize acquisition time (∼4× less time in some cases while providing similar chemical image accuracy), thus reducing stress on plants during in situ LMJ-SSP-MS analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 14521-14525 |
| Number of pages | 5 |
| Journal | Analytical Chemistry |
| Volume | 95 |
| Issue number | 39 |
| DOIs | |
| State | Published - Oct 3 2023 |
Funding
This work and all authors were supported by the U.S. Department of Energy, Office of Science, Biological and Environmental Research, Bioimaging Science Program. The fabrication of the microfluidic platforms was carried out in the Nanofabrication Research Laboratory at the Center for Nanophase Materials Sciences, which is a DOE Office of Science User Facility. The 5500 QTRAP instrument used in this work was provided on loan from Sciex through a cooperative research and development agreement. This work and all authors were supported by the U.S. Department of Energy, Office of Science, Biological and Environmental Research, Bioimaging Science Program. The fabrication of the microfluidic platforms was carried out in the Nanofabrication Research Laboratory at the Center for Nanophase Materials Sciences, which is a DOE Office of Science User Facility.
| Funders | Funder number |
|---|---|
| Bioimaging Science Program | |
| Center for Nanophase Materials Sciences | |
| U.S. Department of Energy | |
| Office of Science | |
| Biological and Environmental Research |