Abstract
The structure and dynamics of lipid membranes in the presence of extracellular macromolecules are critical for cell membrane functions and many pharmaceutical applications. The pathogen virulence-suppressing end-phosphorylated polyethylene glycol (PEG) triblock copolymer (Pi-ABAPEG) markedly changes the interactions with lipid vesicle membranes and prevents PEG-induced vesicle phase separation in contrast to the unphosphorylated copolymer (ABAPEG). Pi-ABAPEG weakly absorbs on the surface of lipid vesicle membranes and slightly changes the structure of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) unilamellar vesicles at 37 °C, as evidenced by small angle neutron scattering. X-ray reflectivity measurements confirm the weak adsorption of Pi-ABAPEG on DMPC monolayer, resulting in a more compact DMPC monolayer structure. Neutron spin-echo results show that the adsorption of Pi-ABAPEG on DMPC vesicle membranes increases the membrane bending modulus κ.
| Original language | English |
|---|---|
| Pages (from-to) | 983-989 |
| Number of pages | 7 |
| Journal | Soft Matter |
| Volume | 16 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2020 |
Funding
Work in the Centre for Molecular Engineering and Materials Science Division of Argonne National Laboratory was supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, Materials Science and Engineering Division. M. N. and Y. L. acknowledge funding support of cooperative agreement 70NANB15H259 and 70NANB15H260, respectively, from NIST, U.S. Department of Commerce. Access to the NG3-SANS instrument and NGA-NSE spectrometer was provided by the Center for High Resolution Neutron Scattering, a partnership between NIST and the NSF under Agreement No. DMR-1508249. NSF’s ChemMatCARS Sector 15 is supported by the Divisions of Chemistry (CHE) and Materials Research (DMR), National Science Foundation, under grant number NSF/CHE-1834750. This research used resources of the Advanced Photon Source (APS) and the Centre for Nanoscale Materials (CNM), a U.S. Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357. A portion of the synthesis was conducted at the Centre for Nanophase Materials Sciences (CNMS), which is the DOE Office of Science User Facilities. Part of the SANS was done on the CG-3 Bio-SANS instrument at the High Flux Isotope Reactor (HFIR), which is sponsored by the Office of Biological & Environmental Research in the Department of Energy’s Office of Science. This work benefited from the use of the SasView application, originally developed under NSF award DMR-0520547. SasView contains code developed with funding from the European Union’s Horizon 2020 Research and Innovation Programme under the SINE2020 project, grant agreement no. 654000. In addition, we gratefully acknowledge the computing resources provided on Bebop, a high-performance computing cluster operated by the Laboratory Computing Resource Centre at Argonne National Laboratory.
| Funders | Funder number |
|---|---|
| Centre for Molecular Engineering and Materials Science Division of Argonne National Laboratory | |
| Centre for Nanoscale Materials | |
| DOE Office of Science | |
| Divisions of Chemistry | |
| Materials Research | |
| Materials Science and Engineering Division | 70NANB15H260, 70NANB15H259 |
| Office of Basic Energy Sciences | |
| National Science Foundation | NSF/CHE-1834750, 1508249 |
| U.S. Department of Energy | |
| National Institute of Standards and Technology | |
| U.S. Department of Commerce | |
| Office of Science | |
| Argonne National Laboratory | DMR-0520547 |
| Horizon 2020 Framework Programme | 654000 |