Abstract
An important biomolecule found in plant seeds and tissues, and in eukaryotic cells is myo-inositol-1,2,3,4,5,6-hexakisphosphate (phytate, IP6). Phytate has many roles, including phosphate, myo-inositol, and mineral storage and retrieval in plants, and a number of metabolic roles, not all of which are known. Despite the importance of phytate in biology, structural information is limited. Aside from this report of the potassium phytate structure, K3[H9IP6]·2H2O, only the structures of the sodium and zinc salts have appeared. The potassium structure reveals the importance of metal ion chelation in stabilizing the conformation, and the two previously reported structures support this finding. Potassium ion and hydrogen bond bridges link the interwoven phytate networks throughout the lattice. 1H NMR (800 MHz) titrations show the conformation crossover from the 1a5e to the 5a1e conformation between pH 9 and 10, and detailed 1H deconvolution studies at low pH reveal the underlying pattern assignments for individual protons.
| Original language | English |
|---|---|
| Pages (from-to) | 1870-1874 |
| Number of pages | 5 |
| Journal | European Journal of Inorganic Chemistry |
| Volume | 2019 |
| Issue number | 14 |
| DOIs | |
| State | Published - Apr 16 2019 |
| Externally published | Yes |
Funding
The authors thank the National Science Foundation (CHE-1710535) for support of this work and (CHE-0923449) for the purchase of the X-ray diffractometer. Support for the NMR instrumentation was provided by an NIH Shared Instrumentation Grant #S10OD016360 and COBRE center grant P30 GM110761. The authors also thank Professor Carlos Kremer, for helpful discussion.
Keywords
- Chelates
- NMR spectroscopy
- Phosphorus
- Potassium phytate
- Structure elucidation