TY - JOUR
T1 - Structural evolution of regenerated silk fibroin under shear
T2 - Combined wide- and small-angle x-ray scattering experiments using synchrotron radiation
AU - Rössle, Manfred
AU - Panine, Pierre
AU - Urban, Volker S.
AU - Riekel, Christian
PY - 2004/7
Y1 - 2004/7
N2 - The structural evolution of regenerated Bombyx mori silk fibroin during shearing with a Couette cell has been studied in situ by synchrotron radiation small- and wide-angle x-ray scattering techniques. An elongation of fibroin molecules was observed with increasing shear rate, followed by an aggregation phase. The aggregates were found to be amorphous with β-conformation according to infrared spectroscopy. Scanning x-ray microdiffraction with a 5 μm beam on aggregated material, which had solidified in air, showed silk II reflections and a material with equatorial reflections close to the silk I structure reflections, but with strong differences in reflection intensities. This silk I type material shows up to two low-angle peaks suggesting the presence of water molecules that might be intercalated between hydrogen-bonded sheets.
AB - The structural evolution of regenerated Bombyx mori silk fibroin during shearing with a Couette cell has been studied in situ by synchrotron radiation small- and wide-angle x-ray scattering techniques. An elongation of fibroin molecules was observed with increasing shear rate, followed by an aggregation phase. The aggregates were found to be amorphous with β-conformation according to infrared spectroscopy. Scanning x-ray microdiffraction with a 5 μm beam on aggregated material, which had solidified in air, showed silk II reflections and a material with equatorial reflections close to the silk I structure reflections, but with strong differences in reflection intensities. This silk I type material shows up to two low-angle peaks suggesting the presence of water molecules that might be intercalated between hydrogen-bonded sheets.
KW - Bombyx mori silk fibroin
KW - In situ shearing experiment
KW - Silk I/II formation
KW - Small-angle x-ray scattering
KW - Synchrotron radiation
KW - Wide-angle x-ray scattering
UR - http://www.scopus.com/inward/record.url?scp=3042781559&partnerID=8YFLogxK
U2 - 10.1002/bip.20083
DO - 10.1002/bip.20083
M3 - Article
C2 - 15211500
AN - SCOPUS:3042781559
SN - 0006-3525
VL - 74
SP - 316
EP - 327
JO - Biopolymers
JF - Biopolymers
IS - 4
ER -