Structural Characterization of Nanoparticle-Supported Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron Scattering

Nicolò Paracini; Philipp Gutfreund; Rebecca Welbourn; Juan Francisco Gonzalez-Martinez; Kexin Zhu; Yansong Miao; Nageshwar Yepuri; Tamim A. Darwish; Christopher Garvey; Sarah Waldie; Johan Larsson; Max Wolff; Marité Cárdenas

doi:10.1021/acsami.2c18956

Structural Characterization of Nanoparticle-Supported Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron Scattering

Nicolò Paracini, Philipp Gutfreund, Rebecca Welbourn, Juan Francisco Gonzalez-Martinez, Kexin Zhu, Yansong Miao, Nageshwar Yepuri, Tamim A. Darwish, Christopher Garvey, Sarah Waldie, Johan Larsson, Max Wolff, Marité Cárdenas

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We combined scattering techniques with direct imaging, to gain access to sub-nanometer scale structural information on stable nanoparticle monolayers assembled on silicon crystals in a noncovalent manner using a Langmuir-Schaefer deposition. The structure of supported lipid bilayers formed on the nanoparticle arrays via vesicle fusion was investigated using a combination of grazing incidence X-ray and neutron scattering techniques complemented by fluorescence microscopy imaging. Ordered nanoparticle assemblies were shown to be suitable and stable substrates for the formation of curved and fluid lipid bilayers that retained lateral mobility, as shown by fluorescence recovery after photobleaching and quartz crystal microbalance measurements. Neutron reflectometry revealed the formation of high-coverage lipid bilayers around the spherical particles together with a flat lipid bilayer on the substrate below the nanoparticles. The presence of coexisting flat and curved supported lipid bilayers on the same substrate, combined with the sub-nanometer accuracy and isotopic sensitivity of grazing incidence neutron scattering, provides a promising novel approach to investigate curvature-dependent membrane phenomena on supported lipid bilayers.

Original language	English
Pages (from-to)	3772-3780
Number of pages	9
Journal	ACS Applied Materials and Interfaces
Volume	15
Issue number	3
DOIs	https://doi.org/10.1021/acsami.2c18956
State	Published - Jan 25 2023
Externally published	Yes

Funding

M.C. and N.P. thank the Swedish Research Council for financial support. N.P. acknowledges support from Nordforsk – Nordic Neutron Science Program (Grant 106881). M.C. thanks Wennergren foundation for financial support. The authors thank the ILL for beamtime (D22: doi:10.5291/ILL-DATA.8-02-912, FIGARO: doi:10.5291/ILL-DATA.8-02-889) and Prof. Jaume Torres for access to a Langmuir Trough at Nanyang Technological University. This study was supported by Singapore MOE Tier 3 (MOE2019-T3-1-012) to Y.M. The National Deuteration Facility in Australia is partly funded by The National Collaborative Research Infrastructure Strategy (NCRIS), an Australian Government initiative. M.C. and M.W. thank the Röntgen-Ångstrom Cluster, grant no. 2021-05963. M.C. and N.P. thank the Swedish Research Council for financial support. N.P. acknowledges support from Nordforsk - Nordic Neutron Science Program (Grant 106881). M.C. thanks Wennergren foundation for financial support. The authors thank the ILL for beamtime (D22: doi:10.5291/ILL-DATA.8-02-912, FIGARO: doi:10.5291/ILL-DATA.8-02-889) and Prof. Jaume Torres for access to a Langmuir Trough at Nanyang Technological University. This study was supported by Singapore MOE Tier 3 (MOE2019-T3-1-012) to Y.M. The National Deuteration Facility in Australia is partly funded by The National Collaborative Research Infrastructure Strategy (NCRIS), an Australian Government initiative. M.C. and M.W. thank the Röntgen-Ångstrom Cluster, grant no. 2021-05963.

Funders	Funder number
Nordforsk - Nordic Neutron Science Program
Nordforsk – Nordic Neutron Science Program	106881
Wennergren foundation	:10.5291/ILL-DATA.8-02-889
Nanyang Technological University	2021-05963, MOE2019-T3-1-012
Vetenskapsrådet

Keywords

GISANS
GISAXS
membrane curvature
model membranes
nanoparticle-supported lipid bilayers, nanoSLB
neutron reflectometry

Access to Document

10.1021/acsami.2c18956

Cite this

Paracini, N., Gutfreund, P., Welbourn, R., Gonzalez-Martinez, J. F., Zhu, K., Miao, Y., Yepuri, N., Darwish, T. A., Garvey, C., Waldie, S., Larsson, J., Wolff, M., & Cárdenas, M. (2023). Structural Characterization of Nanoparticle-Supported Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron Scattering. ACS Applied Materials and Interfaces, 15(3), 3772-3780. https://doi.org/10.1021/acsami.2c18956

@article{c043b598464c4d3cbbbe09bc4a6aca88,

title = "Structural Characterization of Nanoparticle-Supported Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron Scattering",

abstract = "Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We combined scattering techniques with direct imaging, to gain access to sub-nanometer scale structural information on stable nanoparticle monolayers assembled on silicon crystals in a noncovalent manner using a Langmuir-Schaefer deposition. The structure of supported lipid bilayers formed on the nanoparticle arrays via vesicle fusion was investigated using a combination of grazing incidence X-ray and neutron scattering techniques complemented by fluorescence microscopy imaging. Ordered nanoparticle assemblies were shown to be suitable and stable substrates for the formation of curved and fluid lipid bilayers that retained lateral mobility, as shown by fluorescence recovery after photobleaching and quartz crystal microbalance measurements. Neutron reflectometry revealed the formation of high-coverage lipid bilayers around the spherical particles together with a flat lipid bilayer on the substrate below the nanoparticles. The presence of coexisting flat and curved supported lipid bilayers on the same substrate, combined with the sub-nanometer accuracy and isotopic sensitivity of grazing incidence neutron scattering, provides a promising novel approach to investigate curvature-dependent membrane phenomena on supported lipid bilayers.",

keywords = "GISANS, GISAXS, membrane curvature, model membranes, nanoparticle-supported lipid bilayers, nanoSLB, neutron reflectometry",

author = "Nicol{\`o} Paracini and Philipp Gutfreund and Rebecca Welbourn and Gonzalez-Martinez, {Juan Francisco} and Kexin Zhu and Yansong Miao and Nageshwar Yepuri and Darwish, {Tamim A.} and Christopher Garvey and Sarah Waldie and Johan Larsson and Max Wolff and Marit{\'e} C{\'a}rdenas",

note = "Publisher Copyright: {\textcopyright} 2023 The Authors. Published by American Chemical Society.",

year = "2023",

month = jan,

day = "25",

doi = "10.1021/acsami.2c18956",

language = "English",

volume = "15",

pages = "3772--3780",

journal = "ACS Applied Materials and Interfaces",

issn = "1944-8244",

publisher = "American Chemical Society",

number = "3",

}

Paracini, N, Gutfreund, P, Welbourn, R, Gonzalez-Martinez, JF, Zhu, K, Miao, Y, Yepuri, N, Darwish, TA, Garvey, C, Waldie, S, Larsson, J, Wolff, M & Cárdenas, M 2023, 'Structural Characterization of Nanoparticle-Supported Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron Scattering', ACS Applied Materials and Interfaces, vol. 15, no. 3, pp. 3772-3780. https://doi.org/10.1021/acsami.2c18956

TY - JOUR

T1 - Structural Characterization of Nanoparticle-Supported Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron Scattering

AU - Paracini, Nicolò

AU - Gutfreund, Philipp

AU - Welbourn, Rebecca

AU - Gonzalez-Martinez, Juan Francisco

AU - Zhu, Kexin

AU - Miao, Yansong

AU - Yepuri, Nageshwar

AU - Darwish, Tamim A.

AU - Garvey, Christopher

AU - Waldie, Sarah

AU - Larsson, Johan

AU - Wolff, Max

AU - Cárdenas, Marité

PY - 2023/1/25

Y1 - 2023/1/25

N2 - Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We combined scattering techniques with direct imaging, to gain access to sub-nanometer scale structural information on stable nanoparticle monolayers assembled on silicon crystals in a noncovalent manner using a Langmuir-Schaefer deposition. The structure of supported lipid bilayers formed on the nanoparticle arrays via vesicle fusion was investigated using a combination of grazing incidence X-ray and neutron scattering techniques complemented by fluorescence microscopy imaging. Ordered nanoparticle assemblies were shown to be suitable and stable substrates for the formation of curved and fluid lipid bilayers that retained lateral mobility, as shown by fluorescence recovery after photobleaching and quartz crystal microbalance measurements. Neutron reflectometry revealed the formation of high-coverage lipid bilayers around the spherical particles together with a flat lipid bilayer on the substrate below the nanoparticles. The presence of coexisting flat and curved supported lipid bilayers on the same substrate, combined with the sub-nanometer accuracy and isotopic sensitivity of grazing incidence neutron scattering, provides a promising novel approach to investigate curvature-dependent membrane phenomena on supported lipid bilayers.

AB - Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We combined scattering techniques with direct imaging, to gain access to sub-nanometer scale structural information on stable nanoparticle monolayers assembled on silicon crystals in a noncovalent manner using a Langmuir-Schaefer deposition. The structure of supported lipid bilayers formed on the nanoparticle arrays via vesicle fusion was investigated using a combination of grazing incidence X-ray and neutron scattering techniques complemented by fluorescence microscopy imaging. Ordered nanoparticle assemblies were shown to be suitable and stable substrates for the formation of curved and fluid lipid bilayers that retained lateral mobility, as shown by fluorescence recovery after photobleaching and quartz crystal microbalance measurements. Neutron reflectometry revealed the formation of high-coverage lipid bilayers around the spherical particles together with a flat lipid bilayer on the substrate below the nanoparticles. The presence of coexisting flat and curved supported lipid bilayers on the same substrate, combined with the sub-nanometer accuracy and isotopic sensitivity of grazing incidence neutron scattering, provides a promising novel approach to investigate curvature-dependent membrane phenomena on supported lipid bilayers.

KW - GISANS

KW - GISAXS

KW - membrane curvature

KW - model membranes

KW - nanoparticle-supported lipid bilayers, nanoSLB

KW - neutron reflectometry

UR - http://www.scopus.com/inward/record.url?scp=85146341807&partnerID=8YFLogxK

U2 - 10.1021/acsami.2c18956

DO - 10.1021/acsami.2c18956

M3 - Article

C2 - 36625710

AN - SCOPUS:85146341807

SN - 1944-8244

VL - 15

SP - 3772

EP - 3780

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 3

ER -

Structural Characterization of Nanoparticle-Supported Lipid Bilayer Arrays by Grazing Incidence X-ray and Neutron Scattering

Abstract

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this