Structural characterization of lignin in wild-type versus COMT down-regulated switchgrass

Reichel Samuel; Yunqiao Pu; Nan Jiang; Chunxiang Fu; Zeng Yu Wang; Arthur Ragauskas

doi:10.3389/fenrg.2013.00014

Structural characterization of lignin in wild-type versus COMT down-regulated switchgrass

Reichel Samuel, Yunqiao Pu, Nan Jiang, Chunxiang Fu, Zeng Yu Wang, Arthur Ragauskas

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

This study examined the chemical structural characteristics of cellulolytic enzyme lignin isolated from switchgrass focusing on comparisons between wild-type control and caffeic acid 3-O-methyltransferase (COMT) down-regulated transgenic line. Nuclear magnetic resonance techniques including ¹³ C, ³¹ P, and two-dimensional ¹³ C- ¹ H heteronuclear single quantum coherence as well as gel permeation chromatography were employed. Compared to the wild-type, the COMT down-regulated transgenic switchgrass lignin demonstrated a decrease in syringyl (S):guaiacyl (G) ratio and p-coumarate:ferulate ratio, an increase in relative abundance of phenylcoumaran unit, and a comparable content of total free phenolic OH groups along with formation of benzodioxane unit. In addition, COMT down-regulation had no significant effects on the lignin molecular weights during its biosynthesis process.

Original language	English
Article number	14
Journal	Frontiers in Energy Research
Volume	1
Issue number	JAN
DOIs	https://doi.org/10.3389/fenrg.2013.00014
State	Published - 2014
Externally published	Yes

Keywords

COMT transgenic
Lignin
Molecular weights
NMR
Switchgrass

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10.3389/fenrg.2013.00014

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title = "Structural characterization of lignin in wild-type versus COMT down-regulated switchgrass",

abstract = " This study examined the chemical structural characteristics of cellulolytic enzyme lignin isolated from switchgrass focusing on comparisons between wild-type control and caffeic acid 3-O-methyltransferase (COMT) down-regulated transgenic line. Nuclear magnetic resonance techniques including 13 C, 31 P, and two-dimensional 13 C- 1 H heteronuclear single quantum coherence as well as gel permeation chromatography were employed. Compared to the wild-type, the COMT down-regulated transgenic switchgrass lignin demonstrated a decrease in syringyl (S):guaiacyl (G) ratio and p-coumarate:ferulate ratio, an increase in relative abundance of phenylcoumaran unit, and a comparable content of total free phenolic OH groups along with formation of benzodioxane unit. In addition, COMT down-regulation had no significant effects on the lignin molecular weights during its biosynthesis process.",

keywords = "COMT transgenic, Lignin, Molecular weights, NMR, Switchgrass",

author = "Reichel Samuel and Yunqiao Pu and Nan Jiang and Chunxiang Fu and Wang, {Zeng Yu} and Arthur Ragauskas",

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doi = "10.3389/fenrg.2013.00014",

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AU - Samuel, Reichel

AU - Pu, Yunqiao

AU - Jiang, Nan

AU - Fu, Chunxiang

AU - Wang, Zeng Yu

AU - Ragauskas, Arthur

PY - 2014

Y1 - 2014

N2 - This study examined the chemical structural characteristics of cellulolytic enzyme lignin isolated from switchgrass focusing on comparisons between wild-type control and caffeic acid 3-O-methyltransferase (COMT) down-regulated transgenic line. Nuclear magnetic resonance techniques including 13 C, 31 P, and two-dimensional 13 C- 1 H heteronuclear single quantum coherence as well as gel permeation chromatography were employed. Compared to the wild-type, the COMT down-regulated transgenic switchgrass lignin demonstrated a decrease in syringyl (S):guaiacyl (G) ratio and p-coumarate:ferulate ratio, an increase in relative abundance of phenylcoumaran unit, and a comparable content of total free phenolic OH groups along with formation of benzodioxane unit. In addition, COMT down-regulation had no significant effects on the lignin molecular weights during its biosynthesis process.

AB - This study examined the chemical structural characteristics of cellulolytic enzyme lignin isolated from switchgrass focusing on comparisons between wild-type control and caffeic acid 3-O-methyltransferase (COMT) down-regulated transgenic line. Nuclear magnetic resonance techniques including 13 C, 31 P, and two-dimensional 13 C- 1 H heteronuclear single quantum coherence as well as gel permeation chromatography were employed. Compared to the wild-type, the COMT down-regulated transgenic switchgrass lignin demonstrated a decrease in syringyl (S):guaiacyl (G) ratio and p-coumarate:ferulate ratio, an increase in relative abundance of phenylcoumaran unit, and a comparable content of total free phenolic OH groups along with formation of benzodioxane unit. In addition, COMT down-regulation had no significant effects on the lignin molecular weights during its biosynthesis process.

KW - COMT transgenic

KW - Lignin

KW - Molecular weights

KW - NMR

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JO - Frontiers in Energy Research

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Structural characterization of lignin in wild-type versus COMT down-regulated switchgrass

Abstract

Keywords

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