Structural characterization and comparison of switchgrass ball-milled lignin before and after dilute acid pretreatment

Reichel Samuel; Yunqiao Pu; Babu Raman; Arthur J. Ragauskas

doi:10.1007/s12010-009-8749-y

Structural characterization and comparison of switchgrass ball-milled lignin before and after dilute acid pretreatment

Reichel Samuel, Yunqiao Pu, Babu Raman, Arthur J. Ragauskas

Biomaterials and Biomass Characterizatio

Research output: Contribution to journal › Article › peer-review

241 Scopus citations

Abstract

To reduce the recalcitrance and enhance enzymatic activity, dilute H ₂SO₄ pretreatment was carried out on Alamo switchgrass (Panicum virgatum). Ball-milled lignin was isolated from switchgrass before and after pretreatment. Its structure was characterized by ¹³C, HSQC, and ³¹P NMR spectroscopy. It was confirmed that ball-milled switchgrass lignin is of HGS type with a considerable amount of p-coumarate and felurate esters of lignin. The major ball-milled lignin interunit was the β-O-4 linkage, and a minor amount of phenylcoumarin, resinol, and spirodienone units were also present. As a result of the acid pretreatment, there was 36% decrease of β-O-4 linkage observed. In addition to these changes, the S/G ratio decreases from 0.80 to 0.53.

Original language	English
Pages (from-to)	62-74
Number of pages	13
Journal	Applied Biochemistry and Biotechnology
Volume	162
Issue number	1
DOIs	https://doi.org/10.1007/s12010-009-8749-y
State	Published - Sep 2010

Funding

Acknowledgement The BioEnergy Science Center (BESC) is a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. The authors would like to gratefully acknowledge the financial support from DOE Office of Biological and Environmental Research through the BioEnergy Science Center (DE-AC05-00OR22725). The authors also would like to thank the valuable discussions of Dr. Sannigrahi and Bassem B. Hallac for this manuscript.

Keywords

Ball-milled lignin
C and P NMR spectroscopy
HSQC
Pretreatment
Switchgrass

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abstract = "To reduce the recalcitrance and enhance enzymatic activity, dilute H 2SO4 pretreatment was carried out on Alamo switchgrass (Panicum virgatum). Ball-milled lignin was isolated from switchgrass before and after pretreatment. Its structure was characterized by 13C, HSQC, and 31P NMR spectroscopy. It was confirmed that ball-milled switchgrass lignin is of HGS type with a considerable amount of p-coumarate and felurate esters of lignin. The major ball-milled lignin interunit was the β-O-4 linkage, and a minor amount of phenylcoumarin, resinol, and spirodienone units were also present. As a result of the acid pretreatment, there was 36% decrease of β-O-4 linkage observed. In addition to these changes, the S/G ratio decreases from 0.80 to 0.53.",

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Structural characterization and comparison of switchgrass ball-milled lignin before and after dilute acid pretreatment

Abstract

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Keywords

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