TY - JOUR
T1 - Strip-based amperometric detection of myeloperoxidase
AU - Windmiller, Joshua R.
AU - Chinnapareddy, Soujanya
AU - Santhosh, Padmanabhan
AU - Halámek, Jan
AU - Chuang, Min Chieh
AU - Bocharova, Vera
AU - Tseng, Ta Feng
AU - Chou, Tzu Yang
AU - Katz, Evgeny
AU - Wang, Joseph
PY - 2010/10
Y1 - 2010/10
N2 - The development of a screen-printed strip-based amperometric biosensor for the determination of myeloperoxidase (MPO) levels is reported. The biosensor utilizes 3,3′,5,5′-tetramethylbenzidine (TMB) as a redox mediator to enable high-sensitivity quantification of physiological levels of MPO. A multivariate parameter optimization was performed. Under the optimal conditions, physiological levels of MPO between 3 and 18. U/L were detected in both acetate buffer (pH 4.5) and human serum using flexible screen-printed electrodes (SPE). The potential interference generated by common serum-based electroactive compounds and a similar peroxidase enzyme was also investigated. The proposed detection methodology offers a simpler, more rapid, and cost-effective alternative to conventional MPO immunoassays, thereby leading to further development in point-of-care testing of acute cardiac events.
AB - The development of a screen-printed strip-based amperometric biosensor for the determination of myeloperoxidase (MPO) levels is reported. The biosensor utilizes 3,3′,5,5′-tetramethylbenzidine (TMB) as a redox mediator to enable high-sensitivity quantification of physiological levels of MPO. A multivariate parameter optimization was performed. Under the optimal conditions, physiological levels of MPO between 3 and 18. U/L were detected in both acetate buffer (pH 4.5) and human serum using flexible screen-printed electrodes (SPE). The potential interference generated by common serum-based electroactive compounds and a similar peroxidase enzyme was also investigated. The proposed detection methodology offers a simpler, more rapid, and cost-effective alternative to conventional MPO immunoassays, thereby leading to further development in point-of-care testing of acute cardiac events.
KW - Amperometry
KW - Biosensor
KW - Myeloperoxidase
KW - Screen-printed electrode
UR - http://www.scopus.com/inward/record.url?scp=77956938309&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2010.07.031
DO - 10.1016/j.bios.2010.07.031
M3 - Article
C2 - 20708401
AN - SCOPUS:77956938309
SN - 0956-5663
VL - 26
SP - 886
EP - 889
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 2
ER -