Strip-based amperometric detection of myeloperoxidase

Joshua R. Windmiller, Soujanya Chinnapareddy, Padmanabhan Santhosh, Jan Halámek, Min Chieh Chuang, Vera Bocharova, Ta Feng Tseng, Tzu Yang Chou, Evgeny Katz, Joseph Wang

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The development of a screen-printed strip-based amperometric biosensor for the determination of myeloperoxidase (MPO) levels is reported. The biosensor utilizes 3,3′,5,5′-tetramethylbenzidine (TMB) as a redox mediator to enable high-sensitivity quantification of physiological levels of MPO. A multivariate parameter optimization was performed. Under the optimal conditions, physiological levels of MPO between 3 and 18. U/L were detected in both acetate buffer (pH 4.5) and human serum using flexible screen-printed electrodes (SPE). The potential interference generated by common serum-based electroactive compounds and a similar peroxidase enzyme was also investigated. The proposed detection methodology offers a simpler, more rapid, and cost-effective alternative to conventional MPO immunoassays, thereby leading to further development in point-of-care testing of acute cardiac events.

Original languageEnglish
Pages (from-to)886-889
Number of pages4
JournalBiosensors and Bioelectronics
Volume26
Issue number2
DOIs
StatePublished - Oct 2010
Externally publishedYes

Keywords

  • Amperometry
  • Biosensor
  • Myeloperoxidase
  • Screen-printed electrode

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