Abstract
A bacterial isolate, B1D3AT, was isolated from river sediment collected from the Hiwassee River near Calhoun, TN, by enrich-ment culturing with a model 5–5′ lignin dimer, dehydrodivanillate, as its sole carbon source. B1D3AT was also shown to utilize several model lignin-derived monomers and dimers as sole carbon sources in a variety of minimal media. Cells were Gram-stain-negative, aerobic, motile, rod-shaped and formed yellow/cream-coloured colonies on rich agar. Optimal growth occurred at 30 °C, pH 7–8, and in the absence of NaCl. The major fatty acids of B1D3AT were C18: 1 ω7c and C17: 1 ω6c. The predominant hydroxy fatty acids were C14: 0 2-OH and C15: 0 2-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidyldimethylethanolamine and sphingoglycolipid. B1D3AT contained spermidine as the only major polyamine. The major isoprenoid quinone was Q-10 with minor amounts of Q-9 and Q-11. The genomic DNA G+C content of B1D3AT was 65.6 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 49 core, universal genes defined by Clusters of Orthologous Groups gene families indicated that B1D3AT was a member of the genus Sphingobium. B1D3AT was most closely related to Sphingobium sp. SYK-6, with a 100 % 16S rRNA gene sequence similarity. B1D3AT showed 78.1–89.9 % average nucleotide identity and 19.5–22.2% digital DNA–DNA hybridization identity with other type strains from the genus Sphingobium. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain B1D3AT should be classified as representing a novel species of the genus Sphingobium, for which the name Sphingobium lignivorans sp. nov. is proposed. The type strain is strain B1D3AT (ATCC TSD-279T=DSM 111877T).
Original language | English |
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Article number | 005704 |
Journal | International Journal of Systematic and Evolutionary Microbiology |
Volume | 73 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1 2023 |
Funding
This manuscript has been authored by UT-Battelle, LLC under Contract No. DE-AC05-00OR22725 with the US Department of Energy. This work was partially supported by the Center for Bioenergy Innovation, a US DOE Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. This material is also based upon work supported by the US Department of Energy, Office of Science, Office of Biological and Environmental Research, though an Early Career Award to J.K.M. The funders played no role in the study design, article preparation, or decision to publish.
Keywords
- SYK-6
- Sphingobium
- lignin degradation