Spatial and temporal measurements of temperature and cell viability in response to nanoparticle-mediated photothermal therapy

Jon R. Whitney, Amanda Rodgers, Erica Harvie, William F. Carswell, Suzy Torti, Alex A. Puretzky, Christopher M. Rouleau, David B. Geohegan, Christopher G. Rylander, Marissa N. Rylander

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Aim: Nanoparticle-enhanced photothermal therapy is a promising alternative to tumor resection. However, quantitative measurements of cellular response to these treatments are limited. This article introduces a Bimodal Enhanced Analysis of Spatiotemporal Temperature (BEAST) algorithm to rapidly determine the viability of cancer cells in vitro following photothermal therapy alone or in combination with nanoparticles. Materials & methods: To illustrate the capability of the BEAST viability algorithm, single wall carbon nanohorns were added to renal cancer (RENCA) cells in vitro and time-dependent spatial temperature maps measured with an infrared camera during laser therapy were correlated with post-treatment cell viability distribution maps obtained by cell-staining fluorescent microscopy. Conclusion: The BEAST viability algorithm accurately and rapidly determined the cell viability as a function of time, space and temperature.

Original languageEnglish
Pages (from-to)1729-1742
Number of pages14
JournalNanomedicine
Volume7
Issue number11
DOIs
StatePublished - Nov 2012

Keywords

  • cancer
  • carbon nanohorn
  • photothermal
  • spatiotemporal
  • viability

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