Sparse sampling image reconstruction in Lissajous trajectory beam-scanning multiphoton microscopy

Andreas C. Geiger; Justin A. Newman; Suhas Sreehari; Shane Z. Sullivan; Charles A. Bouman; Garth J. Simpson

doi:10.1117/12.2253514

Sparse sampling image reconstruction in Lissajous trajectory beam-scanning multiphoton microscopy

Andreas C. Geiger, Justin A. Newman, Suhas Sreehari, Shane Z. Sullivan, Charles A. Bouman, Garth J. Simpson

Radar and Computational Imaging

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

5 Scopus citations

Abstract

Propagation of action potentials arises on millisecond timescales, suggesting the need for advancement of methods capable of commensurate volume rendering for in vivo brain mapping. In practice, beam-scanning multiphoton microscopy is widely used to probe brain function, striking a balance between simplicity and penetration depth. However, conventional beam-scanning platforms generally do not provide access to full volume renderings at the speeds necessary to map propagation of action potentials. By combining a sparse sampling strategy based on Lissajous trajectory microscopy in combination with temporal multiplexing for simultaneous imaging of multiple focal planes, whole volumes of cells are potentially accessible each millisecond.

Original language	English
Title of host publication	High-Speed Biomedical Imaging and Spectroscopy
Subtitle of host publication	Toward Big Data Instrumentation and Management II
Editors	Kevin K. Tsia, Keisuke Goda
Publisher	SPIE
ISBN (Electronic)	9781510605930
DOIs	https://doi.org/10.1117/12.2253514
State	Published - 2017
Event	High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II 2017 - San Francisco, United States Duration: Jan 30 2017 → Feb 1 2017

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10076
ISSN (Print)	1605-7422

Conference

Conference	High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II 2017
Country/Territory	United States
City	San Francisco
Period	01/30/17 → 02/1/17

Funding

The authors gratefully acknowledge support from the NIH Grant Numbers R01GM-103401 and R01GM-103910 from the NIGMS.

Keywords

Lissajous
beam-scanning microscopy
high speed imaging
in vivo microscopy
two-photon excited fluorescence

Access to Document

10.1117/12.2253514

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Geiger, A. C., Newman, J. A., Sreehari, S., Sullivan, S. Z., Bouman, C. A., & Simpson, G. J. (2017). Sparse sampling image reconstruction in Lissajous trajectory beam-scanning multiphoton microscopy. In K. K. Tsia, & K. Goda (Eds.), High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II Article 1007606 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10076). SPIE. https://doi.org/10.1117/12.2253514

Geiger, Andreas C. ; Newman, Justin A. ; Sreehari, Suhas et al. / Sparse sampling image reconstruction in Lissajous trajectory beam-scanning multiphoton microscopy. High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II. editor / Kevin K. Tsia ; Keisuke Goda. SPIE, 2017. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

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title = "Sparse sampling image reconstruction in Lissajous trajectory beam-scanning multiphoton microscopy",

abstract = "Propagation of action potentials arises on millisecond timescales, suggesting the need for advancement of methods capable of commensurate volume rendering for in vivo brain mapping. In practice, beam-scanning multiphoton microscopy is widely used to probe brain function, striking a balance between simplicity and penetration depth. However, conventional beam-scanning platforms generally do not provide access to full volume renderings at the speeds necessary to map propagation of action potentials. By combining a sparse sampling strategy based on Lissajous trajectory microscopy in combination with temporal multiplexing for simultaneous imaging of multiple focal planes, whole volumes of cells are potentially accessible each millisecond.",

keywords = "Lissajous, beam-scanning microscopy, high speed imaging, in vivo microscopy, two-photon excited fluorescence",

author = "Geiger, \{Andreas C.\} and Newman, \{Justin A.\} and Suhas Sreehari and Sullivan, \{Shane Z.\} and Bouman, \{Charles A.\} and Simpson, \{Garth J.\}",

note = "Publisher Copyright: Copyright {\textcopyright} 2017 SPIE.; High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II 2017 ; Conference date: 30-01-2017 Through 01-02-2017",

year = "2017",

doi = "10.1117/12.2253514",

language = "English",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Tsia, \{Kevin K.\} and Keisuke Goda",

booktitle = "High-Speed Biomedical Imaging and Spectroscopy",

}

Geiger, AC, Newman, JA, Sreehari, S, Sullivan, SZ, Bouman, CA & Simpson, GJ 2017, Sparse sampling image reconstruction in Lissajous trajectory beam-scanning multiphoton microscopy. in KK Tsia & K Goda (eds), High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II., 1007606, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10076, SPIE, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II 2017, San Francisco, United States, 01/30/17. https://doi.org/10.1117/12.2253514

Sparse sampling image reconstruction in Lissajous trajectory beam-scanning multiphoton microscopy. / Geiger, Andreas C.; Newman, Justin A.; Sreehari, Suhas et al.
High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II. ed. / Kevin K. Tsia; Keisuke Goda. SPIE, 2017. 1007606 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10076).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Sparse sampling image reconstruction in Lissajous trajectory beam-scanning multiphoton microscopy

AU - Geiger, Andreas C.

AU - Newman, Justin A.

AU - Sreehari, Suhas

AU - Sullivan, Shane Z.

AU - Bouman, Charles A.

AU - Simpson, Garth J.

PY - 2017

Y1 - 2017

N2 - Propagation of action potentials arises on millisecond timescales, suggesting the need for advancement of methods capable of commensurate volume rendering for in vivo brain mapping. In practice, beam-scanning multiphoton microscopy is widely used to probe brain function, striking a balance between simplicity and penetration depth. However, conventional beam-scanning platforms generally do not provide access to full volume renderings at the speeds necessary to map propagation of action potentials. By combining a sparse sampling strategy based on Lissajous trajectory microscopy in combination with temporal multiplexing for simultaneous imaging of multiple focal planes, whole volumes of cells are potentially accessible each millisecond.

AB - Propagation of action potentials arises on millisecond timescales, suggesting the need for advancement of methods capable of commensurate volume rendering for in vivo brain mapping. In practice, beam-scanning multiphoton microscopy is widely used to probe brain function, striking a balance between simplicity and penetration depth. However, conventional beam-scanning platforms generally do not provide access to full volume renderings at the speeds necessary to map propagation of action potentials. By combining a sparse sampling strategy based on Lissajous trajectory microscopy in combination with temporal multiplexing for simultaneous imaging of multiple focal planes, whole volumes of cells are potentially accessible each millisecond.

KW - Lissajous

KW - beam-scanning microscopy

KW - high speed imaging

KW - in vivo microscopy

KW - two-photon excited fluorescence

UR - https://www.scopus.com/pages/publications/85020288424

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DO - 10.1117/12.2253514

M3 - Conference contribution

AN - SCOPUS:85020288424

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - High-Speed Biomedical Imaging and Spectroscopy

A2 - Tsia, Kevin K.

A2 - Goda, Keisuke

PB - SPIE

T2 - High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II 2017

Y2 - 30 January 2017 through 1 February 2017

ER -

Geiger AC, Newman JA, Sreehari S, Sullivan SZ, Bouman CA, Simpson GJ. Sparse sampling image reconstruction in Lissajous trajectory beam-scanning multiphoton microscopy. In Tsia KK, Goda K, editors, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II. SPIE. 2017. 1007606. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2253514

Sparse sampling image reconstruction in Lissajous trajectory beam-scanning multiphoton microscopy

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