Sparse sampling image reconstruction in Lissajous trajectory beam-scanning multiphoton microscopy

Andreas C. Geiger, Justin A. Newman, Suhas Sreehari, Shane Z. Sullivan, Charles A. Bouman, Garth J. Simpson

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

5 Scopus citations

Abstract

Propagation of action potentials arises on millisecond timescales, suggesting the need for advancement of methods capable of commensurate volume rendering for in vivo brain mapping. In practice, beam-scanning multiphoton microscopy is widely used to probe brain function, striking a balance between simplicity and penetration depth. However, conventional beam-scanning platforms generally do not provide access to full volume renderings at the speeds necessary to map propagation of action potentials. By combining a sparse sampling strategy based on Lissajous trajectory microscopy in combination with temporal multiplexing for simultaneous imaging of multiple focal planes, whole volumes of cells are potentially accessible each millisecond.

Original languageEnglish
Title of host publicationHigh-Speed Biomedical Imaging and Spectroscopy
Subtitle of host publicationToward Big Data Instrumentation and Management II
EditorsKevin K. Tsia, Keisuke Goda
PublisherSPIE
ISBN (Electronic)9781510605930
DOIs
StatePublished - 2017
Externally publishedYes
EventHigh-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II 2017 - San Francisco, United States
Duration: Jan 30 2017Feb 1 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10076
ISSN (Print)1605-7422

Conference

ConferenceHigh-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II 2017
Country/TerritoryUnited States
CitySan Francisco
Period01/30/1702/1/17

Funding

The authors gratefully acknowledge support from the NIH Grant Numbers R01GM-103401 and R01GM-103910 from the NIGMS.

FundersFunder number
National Institutes of HealthR01GM-103910, R01GM-103401
National Institutes of Health
National Institute of General Medical Sciences

    Keywords

    • beam-scanning microscopy
    • high speed imaging
    • in vivo microscopy
    • Lissajous
    • two-photon excited fluorescence

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