Solution structure of detergent micelles at conditions relevant to membrane protein crystallization

K. Littrell; V. Urban; D. Tiede; P. Thiyagarajan

doi:10.1107/S002188989901314X

Solution structure of detergent micelles at conditions relevant to membrane protein crystallization

K. Littrell, V. Urban, D. Tiede, P. Thiyagarajan

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

In this study small angle neutron scattering was used to characterize the formation of micelles in aqueous solution of the detergents DMG and SPC as a function of detergent concentration and ionic strength of the solvent.The effects on the micelle structure of the additives glycerol and PEG, alone as well as in combination typical for membrane protein crystallization, were also explored.This study suggests that the micelles are rod-like in form at the concentrations studied. The size of the micelles was observed to increase with increasing ionic strength but decrease with the addition of glycerol or PEG.

Original language	English
Pages (from-to)	577-581
Number of pages	5
Journal	Journal of Applied Crystallography
Volume	33
Issue number	3 I
DOIs	https://doi.org/10.1107/S002188989901314X
State	Published - 2000
Externally published	Yes

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title = "Solution structure of detergent micelles at conditions relevant to membrane protein crystallization",

abstract = "In this study small angle neutron scattering was used to characterize the formation of micelles in aqueous solution of the detergents DMG and SPC as a function of detergent concentration and ionic strength of the solvent.The effects on the micelle structure of the additives glycerol and PEG, alone as well as in combination typical for membrane protein crystallization, were also explored.This study suggests that the micelles are rod-like in form at the concentrations studied. The size of the micelles was observed to increase with increasing ionic strength but decrease with the addition of glycerol or PEG.",

author = "K. Littrell and V. Urban and D. Tiede and P. Thiyagarajan",

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AU - Littrell, K.

AU - Urban, V.

AU - Tiede, D.

AU - Thiyagarajan, P.

PY - 2000

Y1 - 2000

N2 - In this study small angle neutron scattering was used to characterize the formation of micelles in aqueous solution of the detergents DMG and SPC as a function of detergent concentration and ionic strength of the solvent.The effects on the micelle structure of the additives glycerol and PEG, alone as well as in combination typical for membrane protein crystallization, were also explored.This study suggests that the micelles are rod-like in form at the concentrations studied. The size of the micelles was observed to increase with increasing ionic strength but decrease with the addition of glycerol or PEG.

AB - In this study small angle neutron scattering was used to characterize the formation of micelles in aqueous solution of the detergents DMG and SPC as a function of detergent concentration and ionic strength of the solvent.The effects on the micelle structure of the additives glycerol and PEG, alone as well as in combination typical for membrane protein crystallization, were also explored.This study suggests that the micelles are rod-like in form at the concentrations studied. The size of the micelles was observed to increase with increasing ionic strength but decrease with the addition of glycerol or PEG.

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U2 - 10.1107/S002188989901314X

DO - 10.1107/S002188989901314X

M3 - Article

AN - SCOPUS:0034510429

SN - 0021-8898

VL - 33

SP - 577

EP - 581

JO - Journal of Applied Crystallography

JF - Journal of Applied Crystallography

IS - 3 I

ER -

Solution structure of detergent micelles at conditions relevant to membrane protein crystallization

Abstract

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