Solution structure of a biological bimolecular electron transfer complex: Characterization of the photosynthetic reaction center-cytochrome c2 protein complex by small angle neutron scattering

D. M. Tiede, K. Littrell, P. A. Marone, R. Zhang, P. Thiyagarajan

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15 Scopus citations

Abstract

The structures of kinetically distinct electron transfer complexes formed between the photosynthetic reaction center from Rhodobacter sphaeroides R-26, and a water-soluble cytochrome c2 were characterized using small angle neutron scattering, SANS. Reaction center-cytochrome c2 complexes, RC-C, exhibiting predominately single exponential electron transfer kinetics were found to be 1:1 molar complexes, consistent with a low resolution, co-crystal, x-ray structure (Adir et al., 1996), provided that the cofactor separation was adjusted to 14 ± 3 Å. Other RC-C configurations are consistent with SANS data, but are distinguishable by cofactor separation. RC-C preparations exhibiting more complex kinetics were found to have a particle volume markedly greater than that of a 1:1 complex. These results suggest that RC aggregation is associated with the variation in kinetics reported in the literature, and provide evidence that the model for the 1:1 complex in co-crystals is relevant to the solution environment.

Original languageEnglish
Pages (from-to)560-564
Number of pages5
JournalJournal of Applied Crystallography
Volume33
Issue number3 I
DOIs
StatePublished - 2000
Externally publishedYes

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