TY - JOUR
T1 - Soft X-ray microscopy with a cryo scanning transmission X-ray microscope
T2 - I. Instrumentation, imaging and spectroscopy
AU - Maser, J.
AU - Osanna, A.
AU - Wang, Y.
AU - Jacobsen, C.
AU - Kirz, J.
AU - Spector, S.
AU - Winn, B.
AU - Tennant, D.
PY - 2000
Y1 - 2000
N2 - We have developed a cryo scanning transmission X-ray microscope which uses soft X-rays from the National Synchrotron Light Source. The system is capable of imaging frozen hydrated specimens with a thickness of up to 10 μm at temperatures of around 100 K. We show images and spectra from frozen hydrated eukaryotic cells, and a demonstration that biological specimens do not suffer mass loss or morphological changes at radiation doses up to about 1010 Gray. This makes possible studies where multiple images of the same specimen area are needed, such as tomography (Wang et al. (2000) Soft X-ray microscopy with a cryo scanning transmission X-ray microscope: II. Tomography. J. Microsc. 197, 80-93) or spectroscopic analysis.
AB - We have developed a cryo scanning transmission X-ray microscope which uses soft X-rays from the National Synchrotron Light Source. The system is capable of imaging frozen hydrated specimens with a thickness of up to 10 μm at temperatures of around 100 K. We show images and spectra from frozen hydrated eukaryotic cells, and a demonstration that biological specimens do not suffer mass loss or morphological changes at radiation doses up to about 1010 Gray. This makes possible studies where multiple images of the same specimen area are needed, such as tomography (Wang et al. (2000) Soft X-ray microscopy with a cryo scanning transmission X-ray microscope: II. Tomography. J. Microsc. 197, 80-93) or spectroscopic analysis.
KW - Cryo microscopy
KW - Frozen hydrated
KW - Soft X-ray microscopy
KW - Spectromicroscopy
KW - Synchrotron radiation
KW - Zone plate
UR - https://www.scopus.com/pages/publications/0033984979
U2 - 10.1046/j.1365-2818.2000.00630.x
DO - 10.1046/j.1365-2818.2000.00630.x
M3 - Article
C2 - 10620150
AN - SCOPUS:0033984979
SN - 0022-2720
VL - 197
SP - 68
EP - 79
JO - Journal of Microscopy
JF - Journal of Microscopy
IS - 1
ER -