Small-Angle Neutron Scattering Study of a Phosphatidylcholine-Phosphatidylethanolamine Mixture

William T. Heller

doi:10.1021/acsomega.3c04164

Small-Angle Neutron Scattering Study of a Phosphatidylcholine-Phosphatidylethanolamine Mixture

William T. Heller

SANS & Spin Echo

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Abstract

The properties of single-component phospholipid lipid bilayers have been extensively characterized. Natural cell membranes are not so simple, consisting of a diverse mixture of lipids and proteins. While having detailed structural information on complex membranes would be useful for understanding their structure and function, experimentally characterizing such membranes at a level of detail applied to model phospholipid bilayers is challenging. Here, small-angle neutron scattering with selective deuteration was used to characterize a binary lipid mixture composed of 1,2-dimyristoyl-3-sn-glycero-phosphatidylcholine and 1,2-dimyristoyl-3-sn-glycero-phosphatidylethanolamine. The data analysis provided the area per lipid in each leaflet as well as the asymmetry of the composition of the inner and outer leaflets of the bilayer. The results provide new insight into the structure of the lipid bilayer when this lipid mixture is used to prepare vesicles.

Original language	English
Pages (from-to)	33755-33762
Number of pages	8
Journal	ACS Omega
Volume	8
Issue number	37
DOIs	https://doi.org/10.1021/acsomega.3c04164
State	Published - Sep 19 2023

Funding

This research used resources at the Spallation Neutron Source, a DOE Office of Science User Facility operated by the Oak Ridge National Laboratory. The author thanks C. Y. Gao for technical assistance during the neutron scattering experiment and G. Nagy for discussions during the manuscript preparation. Notice of Copyright This manuscript has been authored by UT-Battelle, LLC, under Contract DE-AC05-00OR22725 with the U.S. Department of Energy (DOE). The U.S. Government retains and the publisher, by accepting the article for publication, acknowledges that the U.S. Government retains a nonexclusive, paid-up, irrevocable, worldwide license to publish or reproduce the published form of this manuscript or allow others to do so for U.S. Government purposes. DOE will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan ( http://energy.gov/downloads/doe-public-access-plan ). Acknowledgments

Funders	Funder number
U.S. Department of Energy
Office of Science
Oak Ridge National Laboratory

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abstract = "The properties of single-component phospholipid lipid bilayers have been extensively characterized. Natural cell membranes are not so simple, consisting of a diverse mixture of lipids and proteins. While having detailed structural information on complex membranes would be useful for understanding their structure and function, experimentally characterizing such membranes at a level of detail applied to model phospholipid bilayers is challenging. Here, small-angle neutron scattering with selective deuteration was used to characterize a binary lipid mixture composed of 1,2-dimyristoyl-3-sn-glycero-phosphatidylcholine and 1,2-dimyristoyl-3-sn-glycero-phosphatidylethanolamine. The data analysis provided the area per lipid in each leaflet as well as the asymmetry of the composition of the inner and outer leaflets of the bilayer. The results provide new insight into the structure of the lipid bilayer when this lipid mixture is used to prepare vesicles.",

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AB - The properties of single-component phospholipid lipid bilayers have been extensively characterized. Natural cell membranes are not so simple, consisting of a diverse mixture of lipids and proteins. While having detailed structural information on complex membranes would be useful for understanding their structure and function, experimentally characterizing such membranes at a level of detail applied to model phospholipid bilayers is challenging. Here, small-angle neutron scattering with selective deuteration was used to characterize a binary lipid mixture composed of 1,2-dimyristoyl-3-sn-glycero-phosphatidylcholine and 1,2-dimyristoyl-3-sn-glycero-phosphatidylethanolamine. The data analysis provided the area per lipid in each leaflet as well as the asymmetry of the composition of the inner and outer leaflets of the bilayer. The results provide new insight into the structure of the lipid bilayer when this lipid mixture is used to prepare vesicles.

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Small-Angle Neutron Scattering Study of a Phosphatidylcholine-Phosphatidylethanolamine Mixture

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