Site-specific attachment of gold nanoparticles to DNA templates

K. A. Stevenson, G. Muralidharan, L. Maya, J. C. Wells, J. Barhen, T. Thundat

Research output: Contribution to journalConference articlepeer-review

1 Scopus citations

Abstract

DNA was used as a scaffold for the binding of gold nanoparticles using a standard chemical technique. A DNA template was designed with amino-modified thymines located every 3.7 nm, which would allow the attachment of the carboxylic acid functionalized gold nanoparticles. The gold particles were covalently bound to the amino groups on the DNA using standard 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) chemistry in the presence of a competitor to block excess gold binding sites. The products were analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM).

Original languageEnglish
Pages (from-to)C421-C424
JournalMaterials Research Society Symposium - Proceedings
Volume635
DOIs
StatePublished - 2001
EventAnisotropic Nanoparticles-Synthesis, Characterization and Applications - Boston, MA, United States
Duration: Nov 27 2000Nov 29 2000

Funding

This research was supported by the Laboratory Directed Research and Development Program of Oak Ridge National Laboratory. Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the U. S. Department of Energy under contract DE-AC05-00OR22725.

Fingerprint

Dive into the research topics of 'Site-specific attachment of gold nanoparticles to DNA templates'. Together they form a unique fingerprint.

Cite this