Single-molecule mobility in confined and crowded femtolitre chambers

Jason D. Fowlkes, C. Patrick Collier

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The effects of increased crowding and confinement on the mobility of individual fluorescent molecules were studied using Fluorescence Correlation Spectroscopy (FCS) in a microfluidic device with sealable femtolitre-volume chambers, and compared to three dimensional stochastic Monte Carlo simulations. When crowding and the degree of confinement were increased simultaneously, extended correlation times of fluorescent intensity fluctuations were observed with FCS compared to varying either crowding or confinement alone. Both experimental data and simulation suggest these extended correlation times were due to increased fluorophore adsorption-desorption events at the chamber lid in the presence of crowders. The data in increasingly confined and crowded chambers described here captures some of the salient features of crowding in cell-like environments.

Original languageEnglish
Pages (from-to)877-885
Number of pages9
JournalLab on a Chip
Volume13
Issue number5
DOIs
StatePublished - Mar 7 2013

Fingerprint

Dive into the research topics of 'Single-molecule mobility in confined and crowded femtolitre chambers'. Together they form a unique fingerprint.

Cite this