Simultaneous saccharification and fermentation and a consolidated bioprocessing for Hinoki cypress and Eucalyptus after fibrillation by steam and subsequent wet-disk milling

Akio Kumagai; Shunsuke Kawamura; Seung Hwan Lee; Takashi Endo; Miguel Rodriguez; Jonathan R. Mielenz

doi:10.1016/j.biortech.2014.03.110

Simultaneous saccharification and fermentation and a consolidated bioprocessing for Hinoki cypress and Eucalyptus after fibrillation by steam and subsequent wet-disk milling

Akio Kumagai, Shunsuke Kawamura, Seung Hwan Lee, Takashi Endo, Miguel Rodriguez, Jonathan R. Mielenz

Biomaterials and Biomass Characterizatio

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

An advanced pretreatment method that combines steam treatment (ST) with wet disk milling (WDM) was evaluated using two different species of woods, viz., Hinoki cypress (softwood) and Eucalyptus (hardwood). Bioconversion of the pretreated products was performed using enzymatic saccharification via a commercial cellulase mixture and two types of fermentation processing, i.e., yeast-based simultaneous saccharification and fermentation (SSF) and Clostridium thermocellum-based consolidated bioprocessing (CBP). A higher yield of glucose was obtained in the enzymatic saccharification and fermentation products from SSF and CBP with pretreatment consisting of WDM after ST, as compared to either ST or WDM alone. Maximum ethanol production via SSF and CBP were 359.3 and 79.4. mg/g-cellulose from Hinoki cypress, and 299.5 and 73.1. mg/g-cellulose from Eucalyptus, respectively. While the main fermentation product generated in CBP was acetate, the total products yield was 319.9 and 262.0. mg/g-cellulose from Hinoki cypress and Eucalyptus, respectively.

Original language	English
Pages (from-to)	89-95
Number of pages	7
Journal	Bioresource Technology
Volume	162
DOIs	https://doi.org/10.1016/j.biortech.2014.03.110
State	Published - Jun 2014

Funding

This work was supported by Japan–U.S. cooperation project for research and standardization of Clean Energy Technologies. A portion of this work was conducted in the Oak Ridge National Laboratory in Oak Ridge, TN, USA, in laboratories supported by the Bioenergy Science Center (BESC) which is a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science under a U.S. Government contract DE-AC05-00OR22725.

Funders	Funder number
BioEnergy Science Center
DOE Office of Science	DE-AC05-00OR22725
Office of Biological and Environmental Research
U.S. Department of Energy Bioenergy Research Center

Keywords

Consolidated bioprocessing
Enzymatic saccharification
Simultaneous saccharification and fermentation
Steam treatment
Wet disk milling

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10.1016/j.biortech.2014.03.110

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title = "Simultaneous saccharification and fermentation and a consolidated bioprocessing for Hinoki cypress and Eucalyptus after fibrillation by steam and subsequent wet-disk milling",

abstract = "An advanced pretreatment method that combines steam treatment (ST) with wet disk milling (WDM) was evaluated using two different species of woods, viz., Hinoki cypress (softwood) and Eucalyptus (hardwood). Bioconversion of the pretreated products was performed using enzymatic saccharification via a commercial cellulase mixture and two types of fermentation processing, i.e., yeast-based simultaneous saccharification and fermentation (SSF) and Clostridium thermocellum-based consolidated bioprocessing (CBP). A higher yield of glucose was obtained in the enzymatic saccharification and fermentation products from SSF and CBP with pretreatment consisting of WDM after ST, as compared to either ST or WDM alone. Maximum ethanol production via SSF and CBP were 359.3 and 79.4. mg/g-cellulose from Hinoki cypress, and 299.5 and 73.1. mg/g-cellulose from Eucalyptus, respectively. While the main fermentation product generated in CBP was acetate, the total products yield was 319.9 and 262.0. mg/g-cellulose from Hinoki cypress and Eucalyptus, respectively.",

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author = "Akio Kumagai and Shunsuke Kawamura and Lee, {Seung Hwan} and Takashi Endo and Miguel Rodriguez and Mielenz, {Jonathan R.}",

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AU - Endo, Takashi

AU - Rodriguez, Miguel

AU - Mielenz, Jonathan R.

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Simultaneous saccharification and fermentation and a consolidated bioprocessing for Hinoki cypress and Eucalyptus after fibrillation by steam and subsequent wet-disk milling

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Keywords

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