Abstract
A simple sensor for viral particles based on ionic conductivity through anodized alumina membranes was demonstrated using MS2 bacteriophage as an example. A facile two-point measuring scheme is geared toward realization using a computer's sound card input/output capabilities suitable for a fast and inexpensive point of care testing. The lowest detection concentration down to ∼7 pfu/mL and a large dynamic range up to ∼2000 pfu/mL were obtained due to physical optimization that included proper length and diameter for the pores, removing the oxide layer at the electrode, as well as the chemical optimization of covalent binding of antibodies to the pore's walls.
| Original language | English |
|---|---|
| Pages (from-to) | 488-492 |
| Number of pages | 5 |
| Journal | ACS Sensors |
| Volume | 1 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 27 2016 |
Funding
This work was partially supported by grant from the National Institute of Health (R15-EB-016401-01). The authors are grateful to Dr. P. Cook for help with SEM and Dr. C. Bruce for lending some equipment. A portion of this research was conducted at the Center for Nanophase Materials Sciences, which is a DOE Office of Science User Facility.
| Funders | Funder number |
|---|---|
| National Institutes of Health | R15-EB-016401-01 |
| Office of Science |
Keywords
- AAO
- MS2
- biosensor
- ionic conductance
- virus sensor
- volume exclusion effect